Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23318 | 5' | -52 | NC_005259.1 | + | 9956 | 0.66 | 0.907691 |
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Target: 5'- aAUCGAGCCgACcGCCCgaccaccgcaccgGUCGAGGu -3' miRNA: 3'- aUGGCUCGGaUGaUGGGaa-----------CAGUUCU- -5' |
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| 23318 | 5' | -52 | NC_005259.1 | + | 21301 | 0.66 | 0.883253 |
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Target: 5'- gGCCGAacgggaGaCCUA-UGCCCgUGUCAAGAu -3' miRNA: 3'- aUGGCU------C-GGAUgAUGGGaACAGUUCU- -5' |
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| 23318 | 5' | -52 | NC_005259.1 | + | 58085 | 0.66 | 0.875466 |
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Target: 5'- aGCuCGGGCCagccCUGCUCUUG-CAGGAu -3' miRNA: 3'- aUG-GCUCGGau--GAUGGGAACaGUUCU- -5' |
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| 23318 | 5' | -52 | NC_005259.1 | + | 64443 | 0.67 | 0.832759 |
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Target: 5'- cUGCCGGGgC-GCUGCCCUUGgggCGGcGAa -3' miRNA: 3'- -AUGGCUCgGaUGAUGGGAACa--GUU-CU- -5' |
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| 23318 | 5' | -52 | NC_005259.1 | + | 37987 | 0.68 | 0.784586 |
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Target: 5'- gUACUGcGCCUGCgUGCCCUUccaCAAGAu -3' miRNA: 3'- -AUGGCuCGGAUG-AUGGGAAca-GUUCU- -5' |
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| 23318 | 5' | -52 | NC_005259.1 | + | 45559 | 0.69 | 0.743017 |
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Target: 5'- cGCCGGGCC----ACCCUUGcccgCGAGAa -3' miRNA: 3'- aUGGCUCGGaugaUGGGAACa---GUUCU- -5' |
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| 23318 | 5' | -52 | NC_005259.1 | + | 30366 | 0.69 | 0.732302 |
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Target: 5'- cGCCGAucuGCCcGCUGCCCgg--CGAGAa -3' miRNA: 3'- aUGGCU---CGGaUGAUGGGaacaGUUCU- -5' |
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| 23318 | 5' | -52 | NC_005259.1 | + | 778 | 0.7 | 0.710572 |
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Target: 5'- cGCCGAGgCgGCUGCCCUcgGUUggGu -3' miRNA: 3'- aUGGCUCgGaUGAUGGGAa-CAGuuCu -5' |
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| 23318 | 5' | -52 | NC_005259.1 | + | 68523 | 0.71 | 0.632607 |
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Target: 5'- cUGCCGAGCgaGCUGCCCgccacgcGUCGAc- -3' miRNA: 3'- -AUGGCUCGgaUGAUGGGaa-----CAGUUcu -5' |
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| 23318 | 5' | -52 | NC_005259.1 | + | 12718 | 1.08 | 0.003028 |
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Target: 5'- aUACCGAGCCUACUACCCUUGUCAAGAc -3' miRNA: 3'- -AUGGCUCGGAUGAUGGGAACAGUUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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