Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23323 | 5' | -58.1 | NC_005259.1 | + | 31897 | 0.66 | 0.61971 |
Target: 5'- uCUCaAGGCCCggcgcGAGCGCCucaaucucGUCacgcaCUCGGg -3' miRNA: 3'- -GAG-UCCGGGaa---CUCGUGG--------CAG-----GAGCC- -5' |
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23323 | 5' | -58.1 | NC_005259.1 | + | 34904 | 0.66 | 0.61971 |
Target: 5'- -aCGGGCCgCUcGGGCAUCacacugcugCCUCGGg -3' miRNA: 3'- gaGUCCGG-GAaCUCGUGGca-------GGAGCC- -5' |
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23323 | 5' | -58.1 | NC_005259.1 | + | 68245 | 0.66 | 0.640869 |
Target: 5'- gCUCaAGGaugaccgcagCCUUGcgcucgucguaGGCACCGUCCUCGc -3' miRNA: 3'- -GAG-UCCg---------GGAAC-----------UCGUGGCAGGAGCc -5' |
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23323 | 5' | -58.1 | NC_005259.1 | + | 32404 | 0.67 | 0.546384 |
Target: 5'- gCUC-GGCUCacacguAGCACCGugUCCUCGGg -3' miRNA: 3'- -GAGuCCGGGaac---UCGUGGC--AGGAGCC- -5' |
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23323 | 5' | -58.1 | NC_005259.1 | + | 23209 | 0.67 | 0.567128 |
Target: 5'- gUguGGCCCaaggacGGGUACCGUCCgcUCGa -3' miRNA: 3'- gAguCCGGGaa----CUCGUGGCAGG--AGCc -5' |
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23323 | 5' | -58.1 | NC_005259.1 | + | 46467 | 0.68 | 0.495715 |
Target: 5'- gCUCAGaUCCUUGAGCGCUGUggccacgcCCUUGa -3' miRNA: 3'- -GAGUCcGGGAACUCGUGGCA--------GGAGCc -5' |
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23323 | 5' | -58.1 | NC_005259.1 | + | 62879 | 0.68 | 0.514739 |
Target: 5'- -gUAGGCCgCUcggucauUGAGCgacGCCGcuUCCUCGGu -3' miRNA: 3'- gaGUCCGG-GA-------ACUCG---UGGC--AGGAGCC- -5' |
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23323 | 5' | -58.1 | NC_005259.1 | + | 33826 | 0.68 | 0.515749 |
Target: 5'- --gGGGUCCggcgaGAGCuuuaucucGCCGUCCUUGGu -3' miRNA: 3'- gagUCCGGGaa---CUCG--------UGGCAGGAGCC- -5' |
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23323 | 5' | -58.1 | NC_005259.1 | + | 16432 | 0.68 | 0.515749 |
Target: 5'- gUCGagcGGCCCaaaccGGGCACCGUCggcaUCGGu -3' miRNA: 3'- gAGU---CCGGGaa---CUCGUGGCAGg---AGCC- -5' |
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23323 | 5' | -58.1 | NC_005259.1 | + | 52399 | 0.69 | 0.485831 |
Target: 5'- cCUCGGGCCaucgaggugUGGGCGCUcgaCUCGGg -3' miRNA: 3'- -GAGUCCGGga-------ACUCGUGGcagGAGCC- -5' |
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23323 | 5' | -58.1 | NC_005259.1 | + | 42038 | 0.72 | 0.290559 |
Target: 5'- gUCGGGUCgacu-GCGCUGUCCUCGGg -3' miRNA: 3'- gAGUCCGGgaacuCGUGGCAGGAGCC- -5' |
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23323 | 5' | -58.1 | NC_005259.1 | + | 56257 | 0.72 | 0.327244 |
Target: 5'- aUCGGcGgCCUUGAGCGCCG-CCgccCGGc -3' miRNA: 3'- gAGUC-CgGGAACUCGUGGCaGGa--GCC- -5' |
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23323 | 5' | -58.1 | NC_005259.1 | + | 9691 | 0.77 | 0.13849 |
Target: 5'- gUCgAGGUcgCCgcucacUGGGCACCGUCCUCGGg -3' miRNA: 3'- gAG-UCCG--GGa-----ACUCGUGGCAGGAGCC- -5' |
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23323 | 5' | -58.1 | NC_005259.1 | + | 44756 | 0.78 | 0.131281 |
Target: 5'- --gAGGCUCUUGAGCACCGaUCCcgcaUCGGc -3' miRNA: 3'- gagUCCGGGAACUCGUGGC-AGG----AGCC- -5' |
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23323 | 5' | -58.1 | NC_005259.1 | + | 10409 | 1.09 | 0.000749 |
Target: 5'- gCUCAGGCCCUUGAGCACCGUCCUCGGc -3' miRNA: 3'- -GAGUCCGGGAACUCGUGGCAGGAGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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