Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23329 | 3' | -53.1 | NC_005259.1 | + | 35843 | 0.71 | 0.67909 |
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Target: 5'- aCCGcc-CGCGAGCacagGUCCGcCGCCGc -3' miRNA: 3'- gGGCaaaGCGCUUGa---CAGGCuGUGGC- -5' |
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| 23329 | 3' | -53.1 | NC_005259.1 | + | 30743 | 0.71 | 0.668393 |
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Target: 5'- aCCUGagagUUCaUGAACUGcaugCCGACGCCGg -3' miRNA: 3'- -GGGCa---AAGcGCUUGACa---GGCUGUGGC- -5' |
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| 23329 | 3' | -53.1 | NC_005259.1 | + | 14336 | 0.71 | 0.657664 |
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Target: 5'- gCCCGcg-UGCGGGCcG-CCGACGCCa -3' miRNA: 3'- -GGGCaaaGCGCUUGaCaGGCUGUGGc -5' |
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| 23329 | 3' | -53.1 | NC_005259.1 | + | 23100 | 0.71 | 0.65659 |
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Target: 5'- aCCGgucgagauggUCGUGAuugaucuGCUGUCCGGCAgCGa -3' miRNA: 3'- gGGCaa--------AGCGCU-------UGACAGGCUGUgGC- -5' |
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| 23329 | 3' | -53.1 | NC_005259.1 | + | 29846 | 0.74 | 0.488848 |
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Target: 5'- aUCCG-UUCGCucGCUGUCgGGCAUCGa -3' miRNA: 3'- -GGGCaAAGCGcuUGACAGgCUGUGGC- -5' |
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| 23329 | 3' | -53.1 | NC_005259.1 | + | 26386 | 0.76 | 0.402895 |
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Target: 5'- gCCUGUUgcUCGCGAu---UCCGACGCCGg -3' miRNA: 3'- -GGGCAA--AGCGCUugacAGGCUGUGGC- -5' |
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| 23329 | 3' | -53.1 | NC_005259.1 | + | 8033 | 1.1 | 0.002359 |
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Target: 5'- cCCCGUUUCGCGAACUGUCCGACACCGc -3' miRNA: 3'- -GGGCAAAGCGCUUGACAGGCUGUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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