Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2333 | 3' | -57.6 | NC_001416.1 | + | 6393 | 0.66 | 0.50711 |
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Target: 5'- aUUCAGA-GgUGgAGCCGCCaCGGGaaCGGa -3' miRNA: 3'- -AAGUUUaCgACgUCGGCGG-GUCC--GCC- -5' |
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| 2333 | 3' | -57.6 | NC_001416.1 | + | 14605 | 0.66 | 0.475853 |
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Target: 5'- aUCAGGUGCcguUGuCAGCgGCacagCCGGGCGa -3' miRNA: 3'- aAGUUUACG---AC-GUCGgCG----GGUCCGCc -5' |
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| 2333 | 3' | -57.6 | NC_001416.1 | + | 8576 | 0.7 | 0.296972 |
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Target: 5'- cUCAguuGAUGCUGCAGCgcauaGCCCAGcucuuuCGGa -3' miRNA: 3'- aAGU---UUACGACGUCGg----CGGGUCc-----GCC- -5' |
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| 2333 | 3' | -57.6 | NC_001416.1 | + | 12455 | 0.7 | 0.296972 |
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Target: 5'- -gCGAA-GCUGgucuuCAGCCGCCCAGGUc- -3' miRNA: 3'- aaGUUUaCGAC-----GUCGGCGGGUCCGcc -5' |
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| 2333 | 3' | -57.6 | NC_001416.1 | + | 17409 | 0.74 | 0.160957 |
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Target: 5'- gUCAGGUGCUGCA-CCGCU--GGCGGc -3' miRNA: 3'- aAGUUUACGACGUcGGCGGguCCGCC- -5' |
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| 2333 | 3' | -57.6 | NC_001416.1 | + | 4428 | 1.09 | 0.000397 |
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Target: 5'- aUUCAAAUGCUGCAGCCGCCCAGGCGGg -3' miRNA: 3'- -AAGUUUACGACGUCGGCGGGUCCGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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