Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23330 | 3' | -53 | NC_005259.1 | + | 60174 | 0.66 | 0.885894 |
Target: 5'- aCGGGCGGcgaacgccuGCUCGACcgACCGGGc -3' miRNA: 3'- aGCCUGUUuac------CGAGCUGa-UGGCCUa -5' |
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23330 | 3' | -53 | NC_005259.1 | + | 62930 | 0.66 | 0.854084 |
Target: 5'- cUCGGuuugc-GGCUCGcgcuGCUGCCGGGc -3' miRNA: 3'- -AGCCuguuuaCCGAGC----UGAUGGCCUa -5' |
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23330 | 3' | -53 | NC_005259.1 | + | 68842 | 0.66 | 0.852391 |
Target: 5'- gUCGGugAGGUgGGCcaucaugUCGACUugcccgcGCCGGGc -3' miRNA: 3'- -AGCCugUUUA-CCG-------AGCUGA-------UGGCCUa -5' |
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23330 | 3' | -53 | NC_005259.1 | + | 59477 | 0.67 | 0.836742 |
Target: 5'- gUUGGGCAgGAUGGCcCGAUaGCCGaGAg -3' miRNA: 3'- -AGCCUGU-UUACCGaGCUGaUGGC-CUa -5' |
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23330 | 3' | -53 | NC_005259.1 | + | 18149 | 0.67 | 0.818533 |
Target: 5'- aUGGACGAGaucgGGCU-GAC-GCCGGAUc -3' miRNA: 3'- aGCCUGUUUa---CCGAgCUGaUGGCCUA- -5' |
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23330 | 3' | -53 | NC_005259.1 | + | 30252 | 0.67 | 0.798563 |
Target: 5'- gCGGAUc--UGGUUCGACUgacaugcACCGGGc -3' miRNA: 3'- aGCCUGuuuACCGAGCUGA-------UGGCCUa -5' |
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23330 | 3' | -53 | NC_005259.1 | + | 34188 | 0.68 | 0.749136 |
Target: 5'- cCGGGC-AGUGGCUCGGCaa-CGGu- -3' miRNA: 3'- aGCCUGuUUACCGAGCUGaugGCCua -5' |
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23330 | 3' | -53 | NC_005259.1 | + | 4617 | 0.69 | 0.73865 |
Target: 5'- gUCGGGCcGGUGGCgCGAgUuCUGGAUa -3' miRNA: 3'- -AGCCUGuUUACCGaGCUgAuGGCCUA- -5' |
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23330 | 3' | -53 | NC_005259.1 | + | 58562 | 0.72 | 0.53998 |
Target: 5'- cUCGGGCGGuugGGCcggaucgcggcgCGGCUGCCGGGc -3' miRNA: 3'- -AGCCUGUUua-CCGa-----------GCUGAUGGCCUa -5' |
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23330 | 3' | -53 | NC_005259.1 | + | 10344 | 0.74 | 0.448997 |
Target: 5'- cUCGGcgGCAGcgGGCUCGAC-AUCGGGc -3' miRNA: 3'- -AGCC--UGUUuaCCGAGCUGaUGGCCUa -5' |
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23330 | 3' | -53 | NC_005259.1 | + | 6585 | 1.06 | 0.003591 |
Target: 5'- cUCGGACAAAUGGCUCGACUACCGGAUc -3' miRNA: 3'- -AGCCUGUUUACCGAGCUGAUGGCCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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