Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23331 | 3' | -50.2 | NC_005259.1 | + | 30002 | 0.66 | 0.962847 |
Target: 5'- gUGGUCAccACGaCAaggugaccaccgcCGGGGCGGUGg -3' miRNA: 3'- -ACCAGU--UGCaGUaaaa---------GCUCCGCCAC- -5' |
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23331 | 3' | -50.2 | NC_005259.1 | + | 65111 | 0.67 | 0.947217 |
Target: 5'- cGGUCAguGCGUCAccgUCGGcgcaccGGCGGc- -3' miRNA: 3'- aCCAGU--UGCAGUaaaAGCU------CCGCCac -5' |
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23331 | 3' | -50.2 | NC_005259.1 | + | 68831 | 0.67 | 0.931746 |
Target: 5'- cGGUCAAagcaGUCGg---UGAGGUGGg- -3' miRNA: 3'- aCCAGUUg---CAGUaaaaGCUCCGCCac -5' |
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23331 | 3' | -50.2 | NC_005259.1 | + | 9471 | 0.67 | 0.931746 |
Target: 5'- cGGUC-ACGUCAgugUUgucgcgcaUCGAGGCGa-- -3' miRNA: 3'- aCCAGuUGCAGUa--AA--------AGCUCCGCcac -5' |
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23331 | 3' | -50.2 | NC_005259.1 | + | 1539 | 0.67 | 0.931746 |
Target: 5'- cGGcaucaUCGGgGcCGcgUUCGGGGCGGUGa -3' miRNA: 3'- aCC-----AGUUgCaGUaaAAGCUCCGCCAC- -5' |
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23331 | 3' | -50.2 | NC_005259.1 | + | 5271 | 0.68 | 0.920003 |
Target: 5'- cGGUCAGuguccCGUCGUUgacaUCGAGucacCGGUGg -3' miRNA: 3'- aCCAGUU-----GCAGUAAa---AGCUCc---GCCAC- -5' |
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23331 | 3' | -50.2 | NC_005259.1 | + | 41105 | 0.68 | 0.913702 |
Target: 5'- cGGUCGGCGgUAUga-CGAugucGGCGGUGc -3' miRNA: 3'- aCCAGUUGCaGUAaaaGCU----CCGCCAC- -5' |
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23331 | 3' | -50.2 | NC_005259.1 | + | 9189 | 0.68 | 0.907116 |
Target: 5'- aUGGUCGAgGUCGUcauaCGGGGcCGGa- -3' miRNA: 3'- -ACCAGUUgCAGUAaaa-GCUCC-GCCac -5' |
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23331 | 3' | -50.2 | NC_005259.1 | + | 54951 | 0.68 | 0.900248 |
Target: 5'- uUGGUCGacGCGUCAUcgUCGucaAGGCGcucGUGc -3' miRNA: 3'- -ACCAGU--UGCAGUAaaAGC---UCCGC---CAC- -5' |
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23331 | 3' | -50.2 | NC_005259.1 | + | 9019 | 0.69 | 0.875631 |
Target: 5'- gGGUCAGaCGuacgcggugcucgaUCAUgugCGGGGCGGUa -3' miRNA: 3'- aCCAGUU-GC--------------AGUAaaaGCUCCGCCAc -5' |
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23331 | 3' | -50.2 | NC_005259.1 | + | 29582 | 0.71 | 0.797896 |
Target: 5'- aGGUCGAUaagGUCAUUgcCGAGGCaauugcauccGGUGg -3' miRNA: 3'- aCCAGUUG---CAGUAAaaGCUCCG----------CCAC- -5' |
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23331 | 3' | -50.2 | NC_005259.1 | + | 58777 | 0.71 | 0.777853 |
Target: 5'- cGGgcgCuguGCGcCGg--UCGAGGCGGUGa -3' miRNA: 3'- aCCa--Gu--UGCaGUaaaAGCUCCGCCAC- -5' |
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23331 | 3' | -50.2 | NC_005259.1 | + | 58922 | 0.71 | 0.757183 |
Target: 5'- cGGUCuuguCGUCGg---CGGGcGCGGUGg -3' miRNA: 3'- aCCAGuu--GCAGUaaaaGCUC-CGCCAC- -5' |
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23331 | 3' | -50.2 | NC_005259.1 | + | 42396 | 0.72 | 0.735983 |
Target: 5'- cGGUCcaugaguCGUCGUacUCGAuGGCGGUGc -3' miRNA: 3'- aCCAGuu-----GCAGUAaaAGCU-CCGCCAC- -5' |
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23331 | 3' | -50.2 | NC_005259.1 | + | 17341 | 0.79 | 0.350258 |
Target: 5'- cGGUCAACGUCGccgUCGAGGuCGGc- -3' miRNA: 3'- aCCAGUUGCAGUaaaAGCUCC-GCCac -5' |
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23331 | 3' | -50.2 | NC_005259.1 | + | 6387 | 1.09 | 0.004727 |
Target: 5'- aUGGUCAACGUCAUUUUCGAGGCGGUGc -3' miRNA: 3'- -ACCAGUUGCAGUAAAAGCUCCGCCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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