Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23333 | 3' | -55.7 | NC_005259.1 | + | 58937 | 0.66 | 0.775384 |
Target: 5'- cGGGCGCGgugGCAg---CGUCGgCCUUGg -3' miRNA: 3'- uCUUGCGCa--CGUacgaGCAGC-GGAGC- -5' |
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23333 | 3' | -55.7 | NC_005259.1 | + | 6565 | 0.66 | 0.775384 |
Target: 5'- --uGCGCG-GCGUGuCagGcCGCCUCGg -3' miRNA: 3'- ucuUGCGCaCGUAC-GagCaGCGGAGC- -5' |
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23333 | 3' | -55.7 | NC_005259.1 | + | 56301 | 0.66 | 0.765468 |
Target: 5'- cAGAccACGUG-GCcgGaccCUCGUCGCCgUCGa -3' miRNA: 3'- -UCU--UGCGCaCGuaC---GAGCAGCGG-AGC- -5' |
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23333 | 3' | -55.7 | NC_005259.1 | + | 50675 | 0.66 | 0.764469 |
Target: 5'- -cAGCGCGUcggugccgacgagGCGcUGCUCGUCGUCgCGc -3' miRNA: 3'- ucUUGCGCA-------------CGU-ACGAGCAGCGGaGC- -5' |
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23333 | 3' | -55.7 | NC_005259.1 | + | 27722 | 0.66 | 0.755421 |
Target: 5'- uGGAACGCGUaCuUGUUCG-CGCC-CGc -3' miRNA: 3'- -UCUUGCGCAcGuACGAGCaGCGGaGC- -5' |
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23333 | 3' | -55.7 | NC_005259.1 | + | 11126 | 0.66 | 0.745252 |
Target: 5'- -uGACG-GUGCGaGC-CGUCGCCUgCGg -3' miRNA: 3'- ucUUGCgCACGUaCGaGCAGCGGA-GC- -5' |
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23333 | 3' | -55.7 | NC_005259.1 | + | 56216 | 0.66 | 0.734972 |
Target: 5'- aGGAuCG-GUGUAUGCcagUCGUCGCCg-- -3' miRNA: 3'- -UCUuGCgCACGUACG---AGCAGCGGagc -5' |
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23333 | 3' | -55.7 | NC_005259.1 | + | 40836 | 0.67 | 0.714126 |
Target: 5'- uGGGcCGCGUGUugAUGCccugCGCCUCGa -3' miRNA: 3'- -UCUuGCGCACG--UACGagcaGCGGAGC- -5' |
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23333 | 3' | -55.7 | NC_005259.1 | + | 14675 | 0.67 | 0.70358 |
Target: 5'- cGGGcCGCGUGCAcucgauucUGCUCGaccccCGCCgCGa -3' miRNA: 3'- -UCUuGCGCACGU--------ACGAGCa----GCGGaGC- -5' |
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23333 | 3' | -55.7 | NC_005259.1 | + | 11801 | 0.68 | 0.650057 |
Target: 5'- cGGGCGCGacccGCuugaGCUCGUCGCUgUCGu -3' miRNA: 3'- uCUUGCGCa---CGua--CGAGCAGCGG-AGC- -5' |
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23333 | 3' | -55.7 | NC_005259.1 | + | 26493 | 0.68 | 0.628466 |
Target: 5'- cGGAACGCc-GCGaGCUCGcCGCC-CGg -3' miRNA: 3'- -UCUUGCGcaCGUaCGAGCaGCGGaGC- -5' |
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23333 | 3' | -55.7 | NC_005259.1 | + | 58690 | 0.68 | 0.606888 |
Target: 5'- ---uCGCGggGUcgGCUCGUCGCCg-- -3' miRNA: 3'- ucuuGCGCa-CGuaCGAGCAGCGGagc -5' |
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23333 | 3' | -55.7 | NC_005259.1 | + | 27442 | 0.69 | 0.574697 |
Target: 5'- --cACGCGUcGCAUgaGCUCGUCGCg-CGg -3' miRNA: 3'- ucuUGCGCA-CGUA--CGAGCAGCGgaGC- -5' |
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23333 | 3' | -55.7 | NC_005259.1 | + | 17330 | 0.69 | 0.564047 |
Target: 5'- cGAGgGUGUGCcgGUcaaCGUCGCCgUCGa -3' miRNA: 3'- uCUUgCGCACGuaCGa--GCAGCGG-AGC- -5' |
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23333 | 3' | -55.7 | NC_005259.1 | + | 61255 | 0.7 | 0.491358 |
Target: 5'- cGggUGCGaGCAUGCgacCGUCGCugguCUCGa -3' miRNA: 3'- uCuuGCGCaCGUACGa--GCAGCG----GAGC- -5' |
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23333 | 3' | -55.7 | NC_005259.1 | + | 67336 | 0.71 | 0.451801 |
Target: 5'- uAGAGCGCGUaccacGUAcGCUCGcCGaCCUCGc -3' miRNA: 3'- -UCUUGCGCA-----CGUaCGAGCaGC-GGAGC- -5' |
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23333 | 3' | -55.7 | NC_005259.1 | + | 50288 | 0.73 | 0.378303 |
Target: 5'- gGGAACGuCGUGCAcgcucUGCcacUCGUCGUaCUCGg -3' miRNA: 3'- -UCUUGC-GCACGU-----ACG---AGCAGCG-GAGC- -5' |
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23333 | 3' | -55.7 | NC_005259.1 | + | 32171 | 0.73 | 0.369696 |
Target: 5'- uAGuAGCGCGUGa--GCUCGcugcgCGCCUCGg -3' miRNA: 3'- -UC-UUGCGCACguaCGAGCa----GCGGAGC- -5' |
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23333 | 3' | -55.7 | NC_005259.1 | + | 5841 | 0.98 | 0.007771 |
Target: 5'- cAGAACGUGcGCAUGCUCGUCGCCUCGg -3' miRNA: 3'- -UCUUGCGCaCGUACGAGCAGCGGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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