Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23336 | 3' | -52.5 | NC_005259.1 | + | 58771 | 0.66 | 0.928023 |
Target: 5'- aCGCUGCGggcgcugUGcgC-CGGUCGAGG-CGg -3' miRNA: 3'- -GCGGUGCa------ACuaGuGCCAGCUCUaGC- -5' |
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23336 | 3' | -52.5 | NC_005259.1 | + | 66885 | 0.66 | 0.928023 |
Target: 5'- gCGCCACGgcuUGAUCgugaACGG-CGguAGAUCc -3' miRNA: 3'- -GCGGUGCa--ACUAG----UGCCaGC--UCUAGc -5' |
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23336 | 3' | -52.5 | NC_005259.1 | + | 36414 | 0.66 | 0.926329 |
Target: 5'- gCGCCucaagGGUCAUGGUcugcgcagcgucgcCGAGGUCGa -3' miRNA: 3'- -GCGGugcaaCUAGUGCCA--------------GCUCUAGC- -5' |
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23336 | 3' | -52.5 | NC_005259.1 | + | 10637 | 0.66 | 0.916275 |
Target: 5'- gGCCGaacaGcUUGGccUCGCGGUCGAGcgCc -3' miRNA: 3'- gCGGUg---C-AACU--AGUGCCAGCUCuaGc -5' |
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23336 | 3' | -52.5 | NC_005259.1 | + | 22997 | 0.66 | 0.909997 |
Target: 5'- aCGCCGCcgaGGUCAccgaggacggcuCGGucaUCGAGAUCGa -3' miRNA: 3'- -GCGGUGcaaCUAGU------------GCC---AGCUCUAGC- -5' |
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23336 | 3' | -52.5 | NC_005259.1 | + | 36153 | 0.66 | 0.909997 |
Target: 5'- uCGCCAUGgc---CACGGUgacgaccuugcCGAGGUCGa -3' miRNA: 3'- -GCGGUGCaacuaGUGCCA-----------GCUCUAGC- -5' |
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23336 | 3' | -52.5 | NC_005259.1 | + | 19149 | 0.67 | 0.889573 |
Target: 5'- gGCaCACaccGAUCAgGGUCGAGGUUc -3' miRNA: 3'- gCG-GUGcaaCUAGUgCCAGCUCUAGc -5' |
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23336 | 3' | -52.5 | NC_005259.1 | + | 39154 | 0.68 | 0.856323 |
Target: 5'- uCGCCgACGUUGAaaccgccgaacacgUCGagcaGGUCGAGAaagucaUCGa -3' miRNA: 3'- -GCGG-UGCAACU--------------AGUg---CCAGCUCU------AGC- -5' |
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23336 | 3' | -52.5 | NC_005259.1 | + | 18162 | 0.68 | 0.850494 |
Target: 5'- gGCUgACGccgGAUCugGcaGUCGAGGUCGa -3' miRNA: 3'- gCGG-UGCaa-CUAGugC--CAGCUCUAGC- -5' |
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23336 | 3' | -52.5 | NC_005259.1 | + | 61877 | 0.68 | 0.850494 |
Target: 5'- uGCCGCG-UGAUCACGaccUCGAuAUCa -3' miRNA: 3'- gCGGUGCaACUAGUGCc--AGCUcUAGc -5' |
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23336 | 3' | -52.5 | NC_005259.1 | + | 66430 | 0.69 | 0.805845 |
Target: 5'- cCGCCACG-UGAUgACGGUCucGAaagugccgUCGa -3' miRNA: 3'- -GCGGUGCaACUAgUGCCAGcuCU--------AGC- -5' |
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23336 | 3' | -52.5 | NC_005259.1 | + | 9665 | 0.69 | 0.776845 |
Target: 5'- uGCC-CGUUGuucCAcaugcgcugcCGGUCGAGGUCGc -3' miRNA: 3'- gCGGuGCAACua-GU----------GCCAGCUCUAGC- -5' |
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23336 | 3' | -52.5 | NC_005259.1 | + | 6641 | 0.7 | 0.756761 |
Target: 5'- cCGCC-CGUgacGA-CACGGUCGAGggUGu -3' miRNA: 3'- -GCGGuGCAa--CUaGUGCCAGCUCuaGC- -5' |
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23336 | 3' | -52.5 | NC_005259.1 | + | 46308 | 0.7 | 0.756761 |
Target: 5'- gGCUGCGUUGuUCGCGGcccCGAGgGUCGc -3' miRNA: 3'- gCGGUGCAACuAGUGCCa--GCUC-UAGC- -5' |
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23336 | 3' | -52.5 | NC_005259.1 | + | 33318 | 0.7 | 0.755744 |
Target: 5'- cCGCCGuCGgcGGUCACGcGgaagugaUCGGGGUCGa -3' miRNA: 3'- -GCGGU-GCaaCUAGUGC-C-------AGCUCUAGC- -5' |
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23336 | 3' | -52.5 | NC_005259.1 | + | 8919 | 0.7 | 0.750635 |
Target: 5'- gGCCGCGUUcugggcucguuugucGAUCACGGU-GGGcgCGc -3' miRNA: 3'- gCGGUGCAA---------------CUAGUGCCAgCUCuaGC- -5' |
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23336 | 3' | -52.5 | NC_005259.1 | + | 8689 | 0.7 | 0.746527 |
Target: 5'- cCGCCuCGaUG-UCGCuGUCGAGGUCGu -3' miRNA: 3'- -GCGGuGCaACuAGUGcCAGCUCUAGC- -5' |
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23336 | 3' | -52.5 | NC_005259.1 | + | 19671 | 0.7 | 0.72573 |
Target: 5'- cCGCUACauccccGGUCA-GGUCGGGAUCGa -3' miRNA: 3'- -GCGGUGcaa---CUAGUgCCAGCUCUAGC- -5' |
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23336 | 3' | -52.5 | NC_005259.1 | + | 18974 | 0.71 | 0.704568 |
Target: 5'- uCGCCGCGUacAUCGCccGGcaCGAGAUCGg -3' miRNA: 3'- -GCGGUGCAacUAGUG--CCa-GCUCUAGC- -5' |
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23336 | 3' | -52.5 | NC_005259.1 | + | 5554 | 0.71 | 0.693878 |
Target: 5'- gGCCGCGUggggUGGUgACGG-CGAGAcCGu -3' miRNA: 3'- gCGGUGCA----ACUAgUGCCaGCUCUaGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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