Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23338 | 3' | -53.6 | NC_005259.1 | + | 6001 | 0.73 | 0.449945 |
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Target: 5'- --aACGCUCAuccgcgUCGAGAUCGAGCucgauGaGCg -3' miRNA: 3'- cugUGCGAGU------AGCUCUAGCUCGu----C-CG- -5' |
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| 23338 | 3' | -53.6 | NC_005259.1 | + | 42366 | 0.77 | 0.294931 |
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Target: 5'- aGACAUGaUCGUUGAGGaaggCGGGCAGGCc -3' miRNA: 3'- -CUGUGCgAGUAGCUCUa---GCUCGUCCG- -5' |
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| 23338 | 3' | -53.6 | NC_005259.1 | + | 59115 | 0.77 | 0.273528 |
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Target: 5'- aGCACGCgcggCAUCGAGcagagcGUCGAGCucGGCa -3' miRNA: 3'- cUGUGCGa---GUAGCUC------UAGCUCGu-CCG- -5' |
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| 23338 | 3' | -53.6 | NC_005259.1 | + | 53604 | 0.8 | 0.189814 |
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Target: 5'- ---uUGCUCGUCGGGGUC-AGCAGGCg -3' miRNA: 3'- cuguGCGAGUAGCUCUAGcUCGUCCG- -5' |
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| 23338 | 3' | -53.6 | NC_005259.1 | + | 19665 | 0.81 | 0.165844 |
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Target: 5'- gGGCAgcCGCUaCAuccccggucaggUCGGGAUCGAGCAGGCu -3' miRNA: 3'- -CUGU--GCGA-GU------------AGCUCUAGCUCGUCCG- -5' |
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| 23338 | 3' | -53.6 | NC_005259.1 | + | 4208 | 1.11 | 0.001362 |
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Target: 5'- cGACACGCUCAUCGAGAUCGAGCAGGCc -3' miRNA: 3'- -CUGUGCGAGUAGCUCUAGCUCGUCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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