Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23338 | 3' | -53.6 | NC_005259.1 | + | 6001 | 0.73 | 0.449945 |
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Target: 5'- --aACGCUCAuccgcgUCGAGAUCGAGCucgauGaGCg -3' miRNA: 3'- cugUGCGAGU------AGCUCUAGCUCGu----C-CG- -5' |
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| 23338 | 3' | -53.6 | NC_005259.1 | + | 4427 | 0.7 | 0.639174 |
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Target: 5'- cGCGCGCc---CGAGGUCGAGCcgcccgugcgaguGGGCa -3' miRNA: 3'- cUGUGCGaguaGCUCUAGCUCG-------------UCCG- -5' |
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| 23338 | 3' | -53.6 | NC_005259.1 | + | 4208 | 1.11 | 0.001362 |
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Target: 5'- cGACACGCUCAUCGAGAUCGAGCAGGCc -3' miRNA: 3'- -CUGUGCGAGUAGCUCUAGCUCGUCCG- -5' |
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| 23338 | 3' | -53.6 | NC_005259.1 | + | 2120 | 0.68 | 0.737406 |
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Target: 5'- cACACGCUCGcCGAGGUCacGCAGacccGCa -3' miRNA: 3'- cUGUGCGAGUaGCUCUAGcuCGUC----CG- -5' |
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| 23338 | 3' | -53.6 | NC_005259.1 | + | 1578 | 0.71 | 0.585542 |
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Target: 5'- aGCGCGCcccaCAcCGAGGUCGAGgucaGGGCa -3' miRNA: 3'- cUGUGCGa---GUaGCUCUAGCUCg---UCCG- -5' |
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| 23338 | 3' | -53.6 | NC_005259.1 | + | 1326 | 0.69 | 0.694803 |
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Target: 5'- -cCAUGgUCGauuUCGAGAUCGAGCGcaacGGUg -3' miRNA: 3'- cuGUGCgAGU---AGCUCUAGCUCGU----CCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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