Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23339 | 5' | -61.3 | NC_005259.1 | + | 50694 | 0.66 | 0.49809 |
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Target: 5'- aGGcgcugCUCGUCGUCGCgCUUgaGCCGGg -3' miRNA: 3'- aCCaaa--GGGCAGCGGCG-GAG--CGGCCa -5' |
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| 23339 | 5' | -61.3 | NC_005259.1 | + | 53240 | 0.77 | 0.085052 |
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Target: 5'- aGGUUcgcgccgccgacaCCGUCGCCGCCgcugUCGCCGGUg -3' miRNA: 3'- aCCAAag-----------GGCAGCGGCGG----AGCGGCCA- -5' |
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| 23339 | 5' | -61.3 | NC_005259.1 | + | 53715 | 0.69 | 0.324344 |
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Target: 5'- gUGGUca-CCG-CGCCGCUgauggugaugUCGCCGGg -3' miRNA: 3'- -ACCAaagGGCaGCGGCGG----------AGCGGCCa -5' |
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| 23339 | 5' | -61.3 | NC_005259.1 | + | 56221 | 0.67 | 0.441211 |
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Target: 5'- cGGUguaugCCaguCGUCGCCGCCgaccaCGuuGGg -3' miRNA: 3'- aCCAaa---GG---GCAGCGGCGGa----GCggCCa -5' |
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| 23339 | 5' | -61.3 | NC_005259.1 | + | 58695 | 0.78 | 0.071517 |
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Target: 5'- gGGUcggCUCGUCGCCGCCgccgucaccaUCGCCGGg -3' miRNA: 3'- aCCAaa-GGGCAGCGGCGG----------AGCGGCCa -5' |
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| 23339 | 5' | -61.3 | NC_005259.1 | + | 58860 | 0.67 | 0.405404 |
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Target: 5'- cGGgcUCgCCGUCGUCGUCgUCGCCu-- -3' miRNA: 3'- aCCaaAG-GGCAGCGGCGG-AGCGGcca -5' |
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| 23339 | 5' | -61.3 | NC_005259.1 | + | 64191 | 0.69 | 0.309652 |
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Target: 5'- cGGgaucUUCCuCGaUGUCGaCCUCGCCGGUc -3' miRNA: 3'- aCCa---AAGG-GCaGCGGC-GGAGCGGCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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