Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2334 | 3' | -56.8 | NC_001416.1 | + | 1802 | 0.66 | 0.584519 |
Target: 5'- uGGGAGGCGaaaAUUGGCGAaCGuccggauGCUGAa -3' miRNA: 3'- -CCCUCCGCcg-UGACCGUUcGU-------UGACU- -5' |
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2334 | 3' | -56.8 | NC_001416.1 | + | 13801 | 0.66 | 0.584519 |
Target: 5'- aGGAGGUGaucagcCGCUGGCGcauugagcagugcAGCgAACUGAg -3' miRNA: 3'- cCCUCCGCc-----GUGACCGU-------------UCG-UUGACU- -5' |
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2334 | 3' | -56.8 | NC_001416.1 | + | 13244 | 0.67 | 0.514211 |
Target: 5'- aGGAGGCcacGGUACUGGagucguuucuggaaGAGCAcggggGCUGGa -3' miRNA: 3'- cCCUCCG---CCGUGACCg-------------UUCGU-----UGACU- -5' |
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2334 | 3' | -56.8 | NC_001416.1 | + | 20356 | 0.67 | 0.499494 |
Target: 5'- uGGccGCGGUGgaGGCAgacguGGCGGCUGAu -3' miRNA: 3'- cCCucCGCCGUgaCCGU-----UCGUUGACU- -5' |
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2334 | 3' | -56.8 | NC_001416.1 | + | 4815 | 0.67 | 0.499494 |
Target: 5'- cGGGAuGGUGGCGggGGCAuuugacuGC-GCUGAc -3' miRNA: 3'- -CCCU-CCGCCGUgaCCGUu------CGuUGACU- -5' |
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2334 | 3' | -56.8 | NC_001416.1 | + | 16809 | 0.67 | 0.499494 |
Target: 5'- cGGGGGCaGGCACaccgcgccgGGCu-GUGGCUGAu -3' miRNA: 3'- cCCUCCG-CCGUGa--------CCGuuCGUUGACU- -5' |
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2334 | 3' | -56.8 | NC_001416.1 | + | 3723 | 0.69 | 0.386045 |
Target: 5'- uGGuGGCGGCAUacaucgccuucacaaUGGCGcucuGCAGCUGc -3' miRNA: 3'- cCCuCCGCCGUG---------------ACCGUu---CGUUGACu -5' |
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2334 | 3' | -56.8 | NC_001416.1 | + | 7445 | 0.71 | 0.285122 |
Target: 5'- cGGucGUGGCACUGGUgAAGCugcauACUGAu -3' miRNA: 3'- cCCucCGCCGUGACCG-UUCGu----UGACU- -5' |
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2334 | 3' | -56.8 | NC_001416.1 | + | 46295 | 0.73 | 0.214071 |
Target: 5'- cGGAGGCGGUGgUGGCuucacGCAcugACUGAc -3' miRNA: 3'- cCCUCCGCCGUgACCGuu---CGU---UGACU- -5' |
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2334 | 3' | -56.8 | NC_001416.1 | + | 2492 | 0.73 | 0.210669 |
Target: 5'- ---uGGCGGCGCUGcGCAucaguauuucccgcuGGCAGCUGGa -3' miRNA: 3'- cccuCCGCCGUGAC-CGU---------------UCGUUGACU- -5' |
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2334 | 3' | -56.8 | NC_001416.1 | + | 4990 | 1.11 | 0.000391 |
Target: 5'- cGGGAGGCGGCACUGGCAAGCAACUGAc -3' miRNA: 3'- -CCCUCCGCCGUGACCGUUCGUUGACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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