Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23341 | 5' | -54.7 | NC_005259.1 | + | 57008 | 0.66 | 0.804298 |
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Target: 5'- uGCGAGcaCCUCaGCGCGgcUCGGCGc -3' miRNA: 3'- gCGCUUuaGGAGaUGCGCa-GGCCGUu -5' |
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| 23341 | 5' | -54.7 | NC_005259.1 | + | 62671 | 0.66 | 0.804298 |
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Target: 5'- aGCGAGgccacgaccucGUCCUCgagUGCGgugucccaUCCGGCGAu -3' miRNA: 3'- gCGCUU-----------UAGGAGau-GCGC--------AGGCCGUU- -5' |
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| 23341 | 5' | -54.7 | NC_005259.1 | + | 61717 | 0.66 | 0.765019 |
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Target: 5'- uCGCGAuccacaCCUUgucgagcaggUGUGCGUCCGGCGAg -3' miRNA: 3'- -GCGCUuua---GGAG----------AUGCGCAGGCCGUU- -5' |
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| 23341 | 5' | -54.7 | NC_005259.1 | + | 8805 | 0.67 | 0.754835 |
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Target: 5'- gGCGAgGAUCUUCU-CGCGccgggCCGGUGAg -3' miRNA: 3'- gCGCU-UUAGGAGAuGCGCa----GGCCGUU- -5' |
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| 23341 | 5' | -54.7 | NC_005259.1 | + | 17569 | 0.67 | 0.754835 |
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Target: 5'- cCGaCGAGAUCCUCccgACGCuG-CCGcGCAAc -3' miRNA: 3'- -GC-GCUUUAGGAGa--UGCG-CaGGC-CGUU- -5' |
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| 23341 | 5' | -54.7 | NC_005259.1 | + | 68301 | 0.7 | 0.571734 |
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Target: 5'- -uCGGGAUCgg--GCGCGUCCGGCGAc -3' miRNA: 3'- gcGCUUUAGgagaUGCGCAGGCCGUU- -5' |
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| 23341 | 5' | -54.7 | NC_005259.1 | + | 60941 | 0.7 | 0.539591 |
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Target: 5'- gGUGAucGUCUgcaccacaUGCGCGUCCGGCGg -3' miRNA: 3'- gCGCUu-UAGGag------AUGCGCAGGCCGUu -5' |
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| 23341 | 5' | -54.7 | NC_005259.1 | + | 2973 | 0.93 | 0.019239 |
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Target: 5'- gCGCGAAAUCCUCUACGCGUCC-GCAAc -3' miRNA: 3'- -GCGCUUUAGGAGAUGCGCAGGcCGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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