Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23343 | 3' | -54.1 | NC_005259.1 | + | 2707 | 1.05 | 0.002609 |
Target: 5'- gGACGGUUUCACCGCCGAUCUGCUCAAc -3' miRNA: 3'- -CUGCCAAAGUGGCGGCUAGACGAGUU- -5' |
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23343 | 3' | -54.1 | NC_005259.1 | + | 5082 | 0.74 | 0.370679 |
Target: 5'- cGACGGgccggugUCACCGgCGAUCUGCg--- -3' miRNA: 3'- -CUGCCaa-----AGUGGCgGCUAGACGaguu -5' |
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23343 | 3' | -54.1 | NC_005259.1 | + | 11040 | 0.68 | 0.702835 |
Target: 5'- -cCGGguggUCACCgcaugacguGCCGAUCgGCUCAGc -3' miRNA: 3'- cuGCCaa--AGUGG---------CGGCUAGaCGAGUU- -5' |
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23343 | 3' | -54.1 | NC_005259.1 | + | 24121 | 0.7 | 0.58164 |
Target: 5'- gGGCGGggacuacacCGCCGCCGccCUGCUCGc -3' miRNA: 3'- -CUGCCaaa------GUGGCGGCuaGACGAGUu -5' |
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23343 | 3' | -54.1 | NC_005259.1 | + | 26825 | 0.73 | 0.416281 |
Target: 5'- cGGCGGUggUGCCGgggCGGUCUGCUCGGg -3' miRNA: 3'- -CUGCCAaaGUGGCg--GCUAGACGAGUU- -5' |
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23343 | 3' | -54.1 | NC_005259.1 | + | 37729 | 0.69 | 0.647924 |
Target: 5'- --aGGcacCGCCGCCGAcgagCUGCUCGAu -3' miRNA: 3'- cugCCaaaGUGGCGGCUa---GACGAGUU- -5' |
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23343 | 3' | -54.1 | NC_005259.1 | + | 39623 | 0.68 | 0.681001 |
Target: 5'- cGACGGUguacgCGCUGcCCGGUUUGUUCc- -3' miRNA: 3'- -CUGCCAaa---GUGGC-GGCUAGACGAGuu -5' |
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23343 | 3' | -54.1 | NC_005259.1 | + | 41633 | 0.66 | 0.805958 |
Target: 5'- cAUGGUggCGgUGCCGGUCUGCgggCGc -3' miRNA: 3'- cUGCCAaaGUgGCGGCUAGACGa--GUu -5' |
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23343 | 3' | -54.1 | NC_005259.1 | + | 45908 | 0.66 | 0.79629 |
Target: 5'- cGGCGGg--CACCGCUGGUagaccgagGCUCu- -3' miRNA: 3'- -CUGCCaaaGUGGCGGCUAga------CGAGuu -5' |
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23343 | 3' | -54.1 | NC_005259.1 | + | 55907 | 0.73 | 0.406888 |
Target: 5'- -cCGGggUCgcuggccagACCGCUGGUCUGCUCGg -3' miRNA: 3'- cuGCCaaAG---------UGGCGGCUAGACGAGUu -5' |
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23343 | 3' | -54.1 | NC_005259.1 | + | 61168 | 0.72 | 0.44229 |
Target: 5'- cGACGGUguaCGCCucgguauggcccucGCCGGUCUGCUUg- -3' miRNA: 3'- -CUGCCAaa-GUGG--------------CGGCUAGACGAGuu -5' |
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23343 | 3' | -54.1 | NC_005259.1 | + | 61765 | 0.7 | 0.548945 |
Target: 5'- cGGCGGcaaUCACCGCCGcauUCUGCa--- -3' miRNA: 3'- -CUGCCaa-AGUGGCGGCu--AGACGaguu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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