Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23344 | 3' | -55.8 | NC_005259.1 | + | 40045 | 0.72 | 0.402895 |
Target: 5'- gGUCGCcggugaucgggUCGGuCGCGAuGACCCAugGGCCGa -3' miRNA: 3'- gCAGUG-----------AGUC-GUGCU-CUGGGU--UCGGC- -5' |
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23344 | 3' | -55.8 | NC_005259.1 | + | 21184 | 0.72 | 0.393947 |
Target: 5'- gGUCA-UCGGCGCGAG-CaCCAAGCUGc -3' miRNA: 3'- gCAGUgAGUCGUGCUCuG-GGUUCGGC- -5' |
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23344 | 3' | -55.8 | NC_005259.1 | + | 44860 | 0.72 | 0.385128 |
Target: 5'- aGUCGCUCAGaccggcgGCGAGAUCCGAcgcGCCc -3' miRNA: 3'- gCAGUGAGUCg------UGCUCUGGGUU---CGGc -5' |
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23344 | 3' | -55.8 | NC_005259.1 | + | 2505 | 1.09 | 0.001204 |
Target: 5'- uCGUCACUCAGCACGAGACCCAAGCCGa -3' miRNA: 3'- -GCAGUGAGUCGUGCUCUGGGUUCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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