Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23348 | 5' | -55 | NC_005259.1 | + | 55146 | 0.66 | 0.793128 |
Target: 5'- cCGACAagguaGUUGGGUgGCUCACCg--- -3' miRNA: 3'- -GCUGUag---CAACUCGgCGAGUGGacga -5' |
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23348 | 5' | -55 | NC_005259.1 | + | 8331 | 0.66 | 0.793128 |
Target: 5'- cCGACggCGaucAGCCGgUCACgCUGCUg -3' miRNA: 3'- -GCUGuaGCaacUCGGCgAGUG-GACGA- -5' |
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23348 | 5' | -55 | NC_005259.1 | + | 33037 | 0.66 | 0.763463 |
Target: 5'- uCGACAccucaagcUUGccgGGGUCGCUCGCgUGCUu -3' miRNA: 3'- -GCUGU--------AGCaa-CUCGGCGAGUGgACGA- -5' |
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23348 | 5' | -55 | NC_005259.1 | + | 28967 | 0.66 | 0.763463 |
Target: 5'- cCGAgGUCGgUGccgaGGCCGCgagCGCCcGCUa -3' miRNA: 3'- -GCUgUAGCaAC----UCGGCGa--GUGGaCGA- -5' |
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23348 | 5' | -55 | NC_005259.1 | + | 1338 | 0.67 | 0.739898 |
Target: 5'- uCGAgAUCGagcgcaacggugccUacUGGGCCGCUCGCC-GCg -3' miRNA: 3'- -GCUgUAGC--------------A--ACUCGGCGAGUGGaCGa -5' |
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23348 | 5' | -55 | NC_005259.1 | + | 46675 | 0.67 | 0.700864 |
Target: 5'- uCGGCGagcUCGgccUGAGCCGC-CGCC-GCg -3' miRNA: 3'- -GCUGU---AGCa--ACUCGGCGaGUGGaCGa -5' |
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23348 | 5' | -55 | NC_005259.1 | + | 53622 | 0.68 | 0.679356 |
Target: 5'- aGGCGUCGagcggugccgUUG-GCCGCUU-CCUGCa -3' miRNA: 3'- gCUGUAGC----------AACuCGGCGAGuGGACGa -5' |
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23348 | 5' | -55 | NC_005259.1 | + | 11119 | 0.69 | 0.59237 |
Target: 5'- gCGACGgugaCGgugcGAGCCG-UCGCCUGCg -3' miRNA: 3'- -GCUGUa---GCaa--CUCGGCgAGUGGACGa -5' |
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23348 | 5' | -55 | NC_005259.1 | + | 58133 | 0.7 | 0.570771 |
Target: 5'- cCGACcgCGUUGAGCCGgauCUCAUCgaacucgucgGCg -3' miRNA: 3'- -GCUGuaGCAACUCGGC---GAGUGGa---------CGa -5' |
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23348 | 5' | -55 | NC_005259.1 | + | 39127 | 0.7 | 0.549368 |
Target: 5'- aCGACAUCGgUGAGCUGCccgaugugaUCGCCgacGUUg -3' miRNA: 3'- -GCUGUAGCaACUCGGCG---------AGUGGa--CGA- -5' |
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23348 | 5' | -55 | NC_005259.1 | + | 63634 | 0.72 | 0.456939 |
Target: 5'- aGAuCAUCGUggcGAGCUGCUCGCUgGCc -3' miRNA: 3'- gCU-GUAGCAa--CUCGGCGAGUGGaCGa -5' |
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23348 | 5' | -55 | NC_005259.1 | + | 55698 | 0.72 | 0.437524 |
Target: 5'- uCGGCAgccUCGUUGAugGCCGCgagCACCUcGCc -3' miRNA: 3'- -GCUGU---AGCAACU--CGGCGa--GUGGA-CGa -5' |
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23348 | 5' | -55 | NC_005259.1 | + | 44052 | 0.74 | 0.31926 |
Target: 5'- aCGACcggcucgagugcgcuGUCGUUGAGCUucugGCUgACCUGCUc -3' miRNA: 3'- -GCUG---------------UAGCAACUCGG----CGAgUGGACGA- -5' |
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23348 | 5' | -55 | NC_005259.1 | + | 57753 | 0.77 | 0.233817 |
Target: 5'- gCGAUGUUGc-GAGCCGCgagCGCCUGCUg -3' miRNA: 3'- -GCUGUAGCaaCUCGGCGa--GUGGACGA- -5' |
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23348 | 5' | -55 | NC_005259.1 | + | 1179 | 1.09 | 0.001447 |
Target: 5'- cCGACAUCGUUGAGCCGCUCACCUGCUa -3' miRNA: 3'- -GCUGUAGCAACUCGGCGAGUGGACGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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