Results 1 - 20 of 29 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23349 | 3' | -55.4 | NC_005259.1 | + | 53681 | 0.66 | 0.756949 |
Target: 5'- -gGGCAUCGCGAuGGU-GCgACCGGUc -3' miRNA: 3'- caCCGUAGCGCUcUUAgUG-UGGCCGc -5' |
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23349 | 3' | -55.4 | NC_005259.1 | + | 12990 | 0.66 | 0.746761 |
Target: 5'- cGUGGUGcUCGCGu---UCGCACuCGGCu -3' miRNA: 3'- -CACCGU-AGCGCucuuAGUGUG-GCCGc -5' |
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23349 | 3' | -55.4 | NC_005259.1 | + | 5073 | 0.66 | 0.746761 |
Target: 5'- -gGGCcggGUCGaCGGGccGGUguCACCGGCGa -3' miRNA: 3'- caCCG---UAGC-GCUC--UUAguGUGGCCGC- -5' |
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23349 | 3' | -55.4 | NC_005259.1 | + | 19860 | 0.66 | 0.736461 |
Target: 5'- uGUGGCGcUCGCGcccgccGGGAUCGCcgacGCCGcGCc -3' miRNA: 3'- -CACCGU-AGCGC------UCUUAGUG----UGGC-CGc -5' |
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23349 | 3' | -55.4 | NC_005259.1 | + | 2010 | 0.66 | 0.736461 |
Target: 5'- -cGGcCAUCGgGuGg--CGCACCGGCa -3' miRNA: 3'- caCC-GUAGCgCuCuuaGUGUGGCCGc -5' |
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23349 | 3' | -55.4 | NC_005259.1 | + | 67985 | 0.66 | 0.776943 |
Target: 5'- cGUGGCcaccgcCGCGAGcGUCGacuugcccucCugCGGCGg -3' miRNA: 3'- -CACCGua----GCGCUCuUAGU----------GugGCCGC- -5' |
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23349 | 3' | -55.4 | NC_005259.1 | + | 41997 | 0.66 | 0.776943 |
Target: 5'- -cGGCG--GUGAGGAcCACACCguGGCGa -3' miRNA: 3'- caCCGUagCGCUCUUaGUGUGG--CCGC- -5' |
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23349 | 3' | -55.4 | NC_005259.1 | + | 32007 | 0.66 | 0.776943 |
Target: 5'- -gGGUAUCGCGAcGGGagGCAaguuccCCGGCu -3' miRNA: 3'- caCCGUAGCGCU-CUUagUGU------GGCCGc -5' |
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23349 | 3' | -55.4 | NC_005259.1 | + | 41786 | 0.66 | 0.767013 |
Target: 5'- -cGGCAUCgGCGAGcAGUCG-GCaGGCGg -3' miRNA: 3'- caCCGUAG-CGCUC-UUAGUgUGgCCGC- -5' |
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23349 | 3' | -55.4 | NC_005259.1 | + | 27902 | 0.66 | 0.767013 |
Target: 5'- cUGGgGUCGcCGAGAc-CGCGCgGGUGg -3' miRNA: 3'- cACCgUAGC-GCUCUuaGUGUGgCCGC- -5' |
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23349 | 3' | -55.4 | NC_005259.1 | + | 40059 | 0.66 | 0.766013 |
Target: 5'- -gGGUcgGUCGCGAugacccaugggccGAcgCGCACCGaGCGc -3' miRNA: 3'- caCCG--UAGCGCU-------------CUuaGUGUGGC-CGC- -5' |
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23349 | 3' | -55.4 | NC_005259.1 | + | 25528 | 0.66 | 0.756949 |
Target: 5'- cGUGGCAUCGCGuGggU---GCUGGg- -3' miRNA: 3'- -CACCGUAGCGCuCuuAgugUGGCCgc -5' |
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23349 | 3' | -55.4 | NC_005259.1 | + | 54088 | 0.67 | 0.704992 |
Target: 5'- gGUGGCGUCGCGcau----CACCGGgGu -3' miRNA: 3'- -CACCGUAGCGCucuuaguGUGGCCgC- -5' |
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23349 | 3' | -55.4 | NC_005259.1 | + | 14557 | 0.67 | 0.714511 |
Target: 5'- -cGGCAggccgaGCGGGGAccggcgaacaccaUCGCGCUGGCc -3' miRNA: 3'- caCCGUag----CGCUCUU-------------AGUGUGGCCGc -5' |
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23349 | 3' | -55.4 | NC_005259.1 | + | 41551 | 0.67 | 0.715565 |
Target: 5'- -aGuCGUCGCGAGcGUUACACCGcCGg -3' miRNA: 3'- caCcGUAGCGCUCuUAGUGUGGCcGC- -5' |
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23349 | 3' | -55.4 | NC_005259.1 | + | 55862 | 0.67 | 0.730231 |
Target: 5'- cGUGGUGaacucgccgagcgccUCGCcccgaccucGGGggUCGCGCCGGgGu -3' miRNA: 3'- -CACCGU---------------AGCG---------CUCuuAGUGUGGCCgC- -5' |
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23349 | 3' | -55.4 | NC_005259.1 | + | 55058 | 0.68 | 0.618804 |
Target: 5'- gGUGGCAgucugCGCGAGAu---CAgCGGCu -3' miRNA: 3'- -CACCGUa----GCGCUCUuaguGUgGCCGc -5' |
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23349 | 3' | -55.4 | NC_005259.1 | + | 9616 | 0.68 | 0.618804 |
Target: 5'- cGUGGCAUCGaacac--CGCGCCGGUGc -3' miRNA: 3'- -CACCGUAGCgcucuuaGUGUGGCCGC- -5' |
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23349 | 3' | -55.4 | NC_005259.1 | + | 61741 | 0.68 | 0.663197 |
Target: 5'- gGUGuGCGUCcgGCGAGAucugugcggcggcaAUCaccgccgcauucugcACACCGGCGg -3' miRNA: 3'- -CAC-CGUAG--CGCUCU--------------UAG---------------UGUGGCCGC- -5' |
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23349 | 3' | -55.4 | NC_005259.1 | + | 24076 | 0.68 | 0.672902 |
Target: 5'- -cGuGCAcCGCGAGAu---CACCGGCa -3' miRNA: 3'- caC-CGUaGCGCUCUuaguGUGGCCGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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