Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23349 | 3' | -55.4 | NC_005259.1 | + | 50967 | 0.68 | 0.650223 |
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Target: 5'- cGUGGCuGUCccacgcgGCGAGcgguGUCGaguCACCGGCGa -3' miRNA: 3'- -CACCG-UAG-------CGCUCu---UAGU---GUGGCCGC- -5' |
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| 23349 | 3' | -55.4 | NC_005259.1 | + | 53681 | 0.66 | 0.756949 |
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Target: 5'- -gGGCAUCGCGAuGGU-GCgACCGGUc -3' miRNA: 3'- caCCGUAGCGCUcUUAgUG-UGGCCGc -5' |
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| 23349 | 3' | -55.4 | NC_005259.1 | + | 54088 | 0.67 | 0.704992 |
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Target: 5'- gGUGGCGUCGCGcau----CACCGGgGu -3' miRNA: 3'- -CACCGUAGCGCucuuaguGUGGCCgC- -5' |
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| 23349 | 3' | -55.4 | NC_005259.1 | + | 55058 | 0.68 | 0.618804 |
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Target: 5'- gGUGGCAgucugCGCGAGAu---CAgCGGCu -3' miRNA: 3'- -CACCGUa----GCGCUCUuaguGUgGCCGc -5' |
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| 23349 | 3' | -55.4 | NC_005259.1 | + | 55862 | 0.67 | 0.730231 |
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Target: 5'- cGUGGUGaacucgccgagcgccUCGCcccgaccucGGGggUCGCGCCGGgGu -3' miRNA: 3'- -CACCGU---------------AGCG---------CUCuuAGUGUGGCCgC- -5' |
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| 23349 | 3' | -55.4 | NC_005259.1 | + | 61604 | 0.72 | 0.433073 |
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Target: 5'- gGUGGgGUCgGCGGGGAacagCAgACCGGCa -3' miRNA: 3'- -CACCgUAG-CGCUCUUa---GUgUGGCCGc -5' |
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| 23349 | 3' | -55.4 | NC_005259.1 | + | 61741 | 0.68 | 0.663197 |
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Target: 5'- gGUGuGCGUCcgGCGAGAucugugcggcggcaAUCaccgccgcauucugcACACCGGCGg -3' miRNA: 3'- -CAC-CGUAG--CGCUCU--------------UAG---------------UGUGGCCGC- -5' |
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| 23349 | 3' | -55.4 | NC_005259.1 | + | 65104 | 0.68 | 0.655632 |
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Target: 5'- cGUGGCAcggucagUGCGucaccgucggCGCACCGGCGg -3' miRNA: 3'- -CACCGUa------GCGCucuua-----GUGUGGCCGC- -5' |
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| 23349 | 3' | -55.4 | NC_005259.1 | + | 67985 | 0.66 | 0.776943 |
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Target: 5'- cGUGGCcaccgcCGCGAGcGUCGacuugcccucCugCGGCGg -3' miRNA: 3'- -CACCGua----GCGCUCuUAGU----------GugGCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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