Results 1 - 20 of 107 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23350 | 5' | -57.8 | NC_005259.1 | + | 48162 | 0.66 | 0.66826 |
Target: 5'- cUCGcCCGaaACcgccccCGCCGCCGCCGGg -3' miRNA: 3'- uAGCuGGCacUGuu----GCGGUGGCGGCU- -5' |
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23350 | 5' | -57.8 | NC_005259.1 | + | 24287 | 0.66 | 0.66826 |
Target: 5'- cUCGACCucggGAUggUGCUGCUGCgCGAu -3' miRNA: 3'- uAGCUGGca--CUGuuGCGGUGGCG-GCU- -5' |
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23350 | 5' | -57.8 | NC_005259.1 | + | 11864 | 0.66 | 0.66826 |
Target: 5'- cGUCGGCgaaCGUGAC--CGCCGCaccgucccaauCGCCGAc -3' miRNA: 3'- -UAGCUG---GCACUGuuGCGGUG-----------GCGGCU- -5' |
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23350 | 5' | -57.8 | NC_005259.1 | + | 52456 | 0.66 | 0.66826 |
Target: 5'- uUCGGCgGaUGGCAGa-CCACCGCCc- -3' miRNA: 3'- uAGCUGgC-ACUGUUgcGGUGGCGGcu -5' |
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23350 | 5' | -57.8 | NC_005259.1 | + | 56390 | 0.66 | 0.657658 |
Target: 5'- cGUCGGCUGaucgucucGAUggUGCCcggcCCGCCGAg -3' miRNA: 3'- -UAGCUGGCa-------CUGuuGCGGu---GGCGGCU- -5' |
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23350 | 5' | -57.8 | NC_005259.1 | + | 17384 | 0.66 | 0.657658 |
Target: 5'- gGUCGACUGcGACAcUGCCGCCcaGCUa- -3' miRNA: 3'- -UAGCUGGCaCUGUuGCGGUGG--CGGcu -5' |
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23350 | 5' | -57.8 | NC_005259.1 | + | 16624 | 0.66 | 0.657658 |
Target: 5'- --gGGCCGaGGCAggccaccuacgGCGaCGCCGCCGAc -3' miRNA: 3'- uagCUGGCaCUGU-----------UGCgGUGGCGGCU- -5' |
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23350 | 5' | -57.8 | NC_005259.1 | + | 4120 | 0.66 | 0.657658 |
Target: 5'- -gCGGCCucGACGugGCCaagACCGUCGGc -3' miRNA: 3'- uaGCUGGcaCUGUugCGG---UGGCGGCU- -5' |
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23350 | 5' | -57.8 | NC_005259.1 | + | 5022 | 0.66 | 0.657658 |
Target: 5'- gAUCGAuCCGgccccacacGACAGCGCCA-CGuuGAu -3' miRNA: 3'- -UAGCU-GGCa--------CUGUUGCGGUgGCggCU- -5' |
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23350 | 5' | -57.8 | NC_005259.1 | + | 45399 | 0.66 | 0.656596 |
Target: 5'- gGUCGccGCCGagcgcgcUGAUGGcCGCCGCCGCUGc -3' miRNA: 3'- -UAGC--UGGC-------ACUGUU-GCGGUGGCGGCu -5' |
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23350 | 5' | -57.8 | NC_005259.1 | + | 22581 | 0.66 | 0.647034 |
Target: 5'- -cCGAguaCGUGGuCGACGCgaucaagcugcaCGCCGCCGAc -3' miRNA: 3'- uaGCUg--GCACU-GUUGCG------------GUGGCGGCU- -5' |
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23350 | 5' | -57.8 | NC_005259.1 | + | 44503 | 0.66 | 0.647034 |
Target: 5'- --aGACCGgggugcccUGGCcacccACGCCGCCGCCc- -3' miRNA: 3'- uagCUGGC--------ACUGu----UGCGGUGGCGGcu -5' |
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23350 | 5' | -57.8 | NC_005259.1 | + | 53162 | 0.66 | 0.647034 |
Target: 5'- -aCG-CCGaGGCcgaucuCGCCACCGCCGc -3' miRNA: 3'- uaGCuGGCaCUGuu----GCGGUGGCGGCu -5' |
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23350 | 5' | -57.8 | NC_005259.1 | + | 36235 | 0.66 | 0.640653 |
Target: 5'- cUUGGCCagcgcgagcgccugcGUcGGCAGCGCCA-CGCCGGa -3' miRNA: 3'- uAGCUGG---------------CA-CUGUUGCGGUgGCGGCU- -5' |
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23350 | 5' | -57.8 | NC_005259.1 | + | 29927 | 0.66 | 0.636398 |
Target: 5'- cAUCGACgCGUGGC--CGCCACgCGgcCCGGu -3' miRNA: 3'- -UAGCUG-GCACUGuuGCGGUG-GC--GGCU- -5' |
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23350 | 5' | -57.8 | NC_005259.1 | + | 20276 | 0.66 | 0.636398 |
Target: 5'- gAUgGAUCGUGGacGCGCUACCG-CGAg -3' miRNA: 3'- -UAgCUGGCACUguUGCGGUGGCgGCU- -5' |
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23350 | 5' | -57.8 | NC_005259.1 | + | 28209 | 0.66 | 0.636398 |
Target: 5'- -gUGuCCGUaGACGGucgugcCGCCACCGUCGGc -3' miRNA: 3'- uaGCuGGCA-CUGUU------GCGGUGGCGGCU- -5' |
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23350 | 5' | -57.8 | NC_005259.1 | + | 30154 | 0.66 | 0.636398 |
Target: 5'- cUCGACCG-GGC--CGCCuacagGCCGCCa- -3' miRNA: 3'- uAGCUGGCaCUGuuGCGG-----UGGCGGcu -5' |
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23350 | 5' | -57.8 | NC_005259.1 | + | 11412 | 0.66 | 0.635334 |
Target: 5'- uUCGGCuCGUGGCcggugcaacgggGAUGCUugggccgcucgcuGCCGCCGGu -3' miRNA: 3'- uAGCUG-GCACUG------------UUGCGG-------------UGGCGGCU- -5' |
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23350 | 5' | -57.8 | NC_005259.1 | + | 309 | 0.66 | 0.63427 |
Target: 5'- cUCGACCGggggugcguccgGcCAACGCaCACCuCCGAc -3' miRNA: 3'- uAGCUGGCa-----------CuGUUGCG-GUGGcGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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