Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23352 | 3' | -57.6 | NC_005259.1 | + | 19265 | 0.66 | 0.561888 |
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Target: 5'- cGGGCCG-CCCGaUGGaagUCAugACCAc -3' miRNA: 3'- uUCCGGUaGGGC-ACCg--AGUugUGGUu -5' |
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| 23352 | 3' | -57.6 | NC_005259.1 | + | 2010 | 0.67 | 0.508792 |
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Target: 5'- -cGGCCAUCggGUGGCgcacCGGCACCu- -3' miRNA: 3'- uuCCGGUAGggCACCGa---GUUGUGGuu -5' |
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| 23352 | 3' | -57.6 | NC_005259.1 | + | 62602 | 0.67 | 0.50567 |
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Target: 5'- aGAGGCUGUgugagcugacgccgCaCCGUGGC-CAGCACCGc -3' miRNA: 3'- -UUCCGGUA--------------G-GGCACCGaGUUGUGGUu -5' |
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| 23352 | 3' | -57.6 | NC_005259.1 | + | 15146 | 0.67 | 0.498417 |
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Target: 5'- cGGGGCCAcCUCgGUGGC-CGAgGCCGAg -3' miRNA: 3'- -UUCCGGUaGGG-CACCGaGUUgUGGUU- -5' |
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| 23352 | 3' | -57.6 | NC_005259.1 | + | 6577 | 0.67 | 0.488138 |
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Target: 5'- cAGGCCGcCUCGgacaaaUGGCUCGACuACCGGa -3' miRNA: 3'- uUCCGGUaGGGC------ACCGAGUUG-UGGUU- -5' |
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| 23352 | 3' | -57.6 | NC_005259.1 | + | 155 | 0.69 | 0.373824 |
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Target: 5'- uGGGCUcgCCCGgcggGGUcCGGCACCAc -3' miRNA: 3'- uUCCGGuaGGGCa---CCGaGUUGUGGUu -5' |
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| 23352 | 3' | -57.6 | NC_005259.1 | + | 9602 | 0.69 | 0.373824 |
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Target: 5'- -uGGCCGUagagcaCCGUGGCaUCgAACACCGc -3' miRNA: 3'- uuCCGGUAg-----GGCACCG-AG-UUGUGGUu -5' |
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| 23352 | 3' | -57.6 | NC_005259.1 | + | 17849 | 0.7 | 0.365146 |
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Target: 5'- cGAGGCCAcCCCGUGG-UCAGCGauCCu- -3' miRNA: 3'- -UUCCGGUaGGGCACCgAGUUGU--GGuu -5' |
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| 23352 | 3' | -57.6 | NC_005259.1 | + | 18328 | 0.7 | 0.331846 |
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Target: 5'- cGAGGCCGgugcgUUCGUGG-UCGACGCCGAc -3' miRNA: 3'- -UUCCGGUa----GGGCACCgAGUUGUGGUU- -5' |
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| 23352 | 3' | -57.6 | NC_005259.1 | + | 665 | 0.73 | 0.221316 |
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Target: 5'- cGAGGgCAUgCUGUGGCUCAGCAgCGu -3' miRNA: 3'- -UUCCgGUAgGGCACCGAGUUGUgGUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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