Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23352 | 5' | -59.5 | NC_005259.1 | + | 22791 | 0.67 | 0.485903 |
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Target: 5'- uGCaGGUGcUCaCCGAGGGUGCCg-- -3' miRNA: 3'- cUGaCCACaAGcGGCUCCCACGGgcc -5' |
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| 23352 | 5' | -59.5 | NC_005259.1 | + | 32968 | 0.68 | 0.47622 |
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Target: 5'- uGACgcagaauugGGUGccgaUGCCGAGGGUGCCg-- -3' miRNA: 3'- -CUGa--------CCACaa--GCGGCUCCCACGGgcc -5' |
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| 23352 | 5' | -59.5 | NC_005259.1 | + | 36931 | 0.68 | 0.47622 |
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Target: 5'- cGAC-GGUGagugUUGCUGucaccGGGGUGCCCGc -3' miRNA: 3'- -CUGaCCACa---AGCGGC-----UCCCACGGGCc -5' |
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| 23352 | 5' | -59.5 | NC_005259.1 | + | 25937 | 0.68 | 0.42933 |
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Target: 5'- ---cGGUGagCGCaCGccGGUGCCCGGc -3' miRNA: 3'- cugaCCACaaGCG-GCucCCACGGGCC- -5' |
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| 23352 | 5' | -59.5 | NC_005259.1 | + | 68786 | 0.7 | 0.368503 |
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Target: 5'- aGCaGGUcGUaCGCCGGGgcggugucGGUGCCCGGu -3' miRNA: 3'- cUGaCCA-CAaGCGGCUC--------CCACGGGCC- -5' |
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| 23352 | 5' | -59.5 | NC_005259.1 | + | 33648 | 0.77 | 0.1232 |
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Target: 5'- cGCUcGGgaaaUCaGCCGAGGGUGCCCGGc -3' miRNA: 3'- cUGA-CCaca-AG-CGGCUCCCACGGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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