Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23353 | 5' | -55.5 | NC_005259.1 | + | 18721 | 0.7 | 0.552751 |
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Target: 5'- gAUCAUCGGGccgCCCGgcaucgguaagUCGUCGGugGu -3' miRNA: 3'- gUAGUAGCUCua-GGGC-----------AGCGGCCugC- -5' |
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| 23353 | 5' | -55.5 | NC_005259.1 | + | 46509 | 0.7 | 0.531987 |
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Target: 5'- -cUCGUCGuacGAUCCCGcgaGCCGGuCGg -3' miRNA: 3'- guAGUAGCu--CUAGGGCag-CGGCCuGC- -5' |
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| 23353 | 5' | -55.5 | NC_005259.1 | + | 58914 | 0.71 | 0.481437 |
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Target: 5'- -cUCGUCGGcGGUCuuGUCGUCGG-CGg -3' miRNA: 3'- guAGUAGCU-CUAGggCAGCGGCCuGC- -5' |
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| 23353 | 5' | -55.5 | NC_005259.1 | + | 68856 | 0.72 | 0.424049 |
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Target: 5'- cCAUCAugUCGAcuugCCCG-CGCCGGGCGc -3' miRNA: 3'- -GUAGU--AGCUcua-GGGCaGCGGCCUGC- -5' |
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| 23353 | 5' | -55.5 | NC_005259.1 | + | 44965 | 0.73 | 0.405846 |
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Target: 5'- gGUCAUCGGGAUgCCGaggGCCGGuGCGa -3' miRNA: 3'- gUAGUAGCUCUAgGGCag-CGGCC-UGC- -5' |
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| 23353 | 5' | -55.5 | NC_005259.1 | + | 60351 | 0.74 | 0.362556 |
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Target: 5'- gAUCAggUCGAGGcccugcccgaaaUCCCGccaaUCGCCGGACa -3' miRNA: 3'- gUAGU--AGCUCU------------AGGGC----AGCGGCCUGc -5' |
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| 23353 | 5' | -55.5 | NC_005259.1 | + | 7052 | 0.76 | 0.259126 |
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Target: 5'- uCGUUgcgCGAGAUCCUGUCGaCGGGCGg -3' miRNA: 3'- -GUAGua-GCUCUAGGGCAGCgGCCUGC- -5' |
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| 23353 | 5' | -55.5 | NC_005259.1 | + | 34105 | 0.79 | 0.181083 |
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Target: 5'- --aCAUCGAGAaCgUGUCGCCGGGCGc -3' miRNA: 3'- guaGUAGCUCUaGgGCAGCGGCCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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