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miRNA: 3'- -CUGCGa-GCGCCG-----------CCaCCG-GUGCGGCG- -5'
miRNA: 3'- -CUGCGAGCGccGCCACCGGugCGGcg -5'
miRNA: 3'- cuGCGaGC-GCCGCCACcGGUGCGGCG- -5'
miRNA: 3'- cuGCGaGCG---CCGCCACCGGuGCGGCG- -5'
miRNA: 3'- cUGCGag-CGCCGcCACCGGU-GCGGCG- -5'
Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 23354 | 3' | -63.8 | NC_005259.1 | + | 66252 | 0.76 | 0.076544 |
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Target: 5'- cGACGCagCGCGGCccgcgccgggcGGcGGCuCACGCCGCc -3' miRNA: 3'- -CUGCGa-GCGCCG-----------CCaCCG-GUGCGGCG- -5' |
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| 23354 | 3' | -63.8 | NC_005259.1 | + | 44796 | 0.78 | 0.049777 |
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Target: 5'- aGACGCUCGC--CGGUGGCCugGCCc- -3' miRNA: 3'- -CUGCGAGCGccGCCACCGGugCGGcg -5' |
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| 23354 | 3' | -63.8 | NC_005259.1 | + | 7422 | 0.78 | 0.048446 |
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Target: 5'- -uCGC-CGuCGGCGGUGcCCGCGCCGCa -3' miRNA: 3'- cuGCGaGC-GCCGCCACcGGUGCGGCG- -5' |
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| 23354 | 3' | -63.8 | NC_005259.1 | + | 38733 | 0.79 | 0.044661 |
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Target: 5'- -cCGC-CGUaccGGCGGUGGCC-CGCCGCa -3' miRNA: 3'- cuGCGaGCG---CCGCCACCGGuGCGGCG- -5' |
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| 23354 | 3' | -63.8 | NC_005259.1 | + | 67973 | 0.8 | 0.037936 |
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Target: 5'- cGCGCgcaGCGGC-GUGGCCAcCGCCGCg -3' miRNA: 3'- cUGCGag-CGCCGcCACCGGU-GCGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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