Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23354 | 3' | -63.8 | NC_005259.1 | + | 49985 | 0.66 | 0.381488 |
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Target: 5'- cGCGCacggaUCGCGuGCGGacGGCUgcccucgugcacggGCGCUGCg -3' miRNA: 3'- cUGCG-----AGCGC-CGCCa-CCGG--------------UGCGGCG- -5' |
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| 23354 | 3' | -63.8 | NC_005259.1 | + | 57020 | 0.66 | 0.383969 |
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Target: 5'- aGCGCggcucggCGCGGUGG-GGUCGCacucGUCGUa -3' miRNA: 3'- cUGCGa------GCGCCGCCaCCGGUG----CGGCG- -5' |
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| 23354 | 3' | -63.8 | NC_005259.1 | + | 60420 | 0.66 | 0.383969 |
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Target: 5'- -cUGCUCGgGGguggcccaCGGggcGGCCACGCC-Cg -3' miRNA: 3'- cuGCGAGCgCC--------GCCa--CCGGUGCGGcG- -5' |
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| 23354 | 3' | -63.8 | NC_005259.1 | + | 42246 | 0.66 | 0.383969 |
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Target: 5'- cGACcacCUCGCGcugugaGGUGGUCACGUCGa -3' miRNA: 3'- -CUGc--GAGCGCcg----CCACCGGUGCGGCg -5' |
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| 23354 | 3' | -63.8 | NC_005259.1 | + | 16812 | 0.66 | 0.383969 |
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Target: 5'- cGCGCUCGgGGcCGG-GGUCAUGauCCGa -3' miRNA: 3'- cUGCGAGCgCC-GCCaCCGGUGC--GGCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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