miRNA: 3'- cUGCG------AGCGCCGCcaccGGUGCGGCG- -5'
miRNA: 3'- -CUGCga-----GcGCCGCCACCGG-UGCG-GCG- -5'
miRNA: 3'- cUGCGaGCGcCgCC-ACCGGUGCGGCG- -5'
miRNA: 3'- cuGCGAGCGCCGcca---CCGGUGCGGCG- -5'
miRNA: 3'- cUGCGag-CGCCGcCACCGGU-GCGGCG- -5'
Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23354 | 3' | -63.8 | NC_005259.1 | + | 46563 | 0.69 | 0.220205 |
|
Target: 5'- cGCGCcuauggUCGCGGCGucgaCCGCGCCGCc -3' miRNA: 3'- cUGCG------AGCGCCGCcaccGGUGCGGCG- -5' |
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| 23354 | 3' | -63.8 | NC_005259.1 | + | 14062 | 0.7 | 0.204542 |
|
Target: 5'- cGACGagaccagCaCGGCGGUGGCCuACGUgCGCa -3' miRNA: 3'- -CUGCga-----GcGCCGCCACCGG-UGCG-GCG- -5' |
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| 23354 | 3' | -63.8 | NC_005259.1 | + | 26046 | 0.7 | 0.199539 |
|
Target: 5'- aGCGC-CGCcG-GGuUGGCCAUGCCGCc -3' miRNA: 3'- cUGCGaGCGcCgCC-ACCGGUGCGGCG- -5' |
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| 23354 | 3' | -63.8 | NC_005259.1 | + | 46315 | 0.71 | 0.176099 |
|
Target: 5'- -uUGUUCGCGGCcccgagGGUCGCGUCGCg -3' miRNA: 3'- cuGCGAGCGCCGcca---CCGGUGCGGCG- -5' |
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| 23354 | 3' | -63.8 | NC_005259.1 | + | 67973 | 0.8 | 0.037936 |
|
Target: 5'- cGCGCgcaGCGGC-GUGGCCAcCGCCGCg -3' miRNA: 3'- cUGCGag-CGCCGcCACCGGU-GCGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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