Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23357 | 5' | -59.2 | NC_005259.1 | + | 45904 | 0.66 | 0.561307 |
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Target: 5'- -cGGUCGGCGggCAcCGCUGGUagaCCgaGGc -3' miRNA: 3'- uuCCAGCCGCa-GU-GCGACCG---GGaaCU- -5' |
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| 23357 | 5' | -59.2 | NC_005259.1 | + | 45135 | 0.66 | 0.540425 |
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Target: 5'- cGAGGUCGGCaaUC-UGCUGaucgcucaggccGCCCUUGGc -3' miRNA: 3'- -UUCCAGCCGc-AGuGCGAC------------CGGGAACU- -5' |
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| 23357 | 5' | -59.2 | NC_005259.1 | + | 54081 | 0.67 | 0.489498 |
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Target: 5'- uGAGGUCgguGGCGUCGCGCaucaccggGGUCaacgUGAg -3' miRNA: 3'- -UUCCAG---CCGCAGUGCGa-------CCGGga--ACU- -5' |
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| 23357 | 5' | -59.2 | NC_005259.1 | + | 14569 | 0.67 | 0.488502 |
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Target: 5'- cGGGgacCGGCGaacaccaUCGCGCUGGCCaccGAc -3' miRNA: 3'- uUCCa--GCCGC-------AGUGCGACCGGgaaCU- -5' |
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| 23357 | 5' | -59.2 | NC_005259.1 | + | 47891 | 0.68 | 0.395209 |
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Target: 5'- cGGGUCaGCGUCGgcuCGUUGaGCgCCUUGAu -3' miRNA: 3'- uUCCAGcCGCAGU---GCGAC-CG-GGAACU- -5' |
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| 23357 | 5' | -59.2 | NC_005259.1 | + | 17357 | 0.72 | 0.245198 |
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Target: 5'- cGAGGUCGGCGgcucgGCGCUGGUCg-UGGu -3' miRNA: 3'- -UUCCAGCCGCag---UGCGACCGGgaACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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