Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23359 | 3' | -55.2 | NC_005259.1 | + | 13441 | 0.66 | 0.739463 |
Target: 5'- cGCGcuaGGCGUgacCGUCG-GCGCGGCGCUg- -3' miRNA: 3'- -CGC---CCGUA---GUAGCaCGUGCUGUGGua -5' |
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23359 | 3' | -55.2 | NC_005259.1 | + | 10186 | 0.66 | 0.76003 |
Target: 5'- -aGGGCAgcaCAUCGUG-AUGACgACCGg -3' miRNA: 3'- cgCCCGUa--GUAGCACgUGCUG-UGGUa -5' |
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23359 | 3' | -55.2 | NC_005259.1 | + | 16065 | 0.66 | 0.76003 |
Target: 5'- -aGGGCAgcgCAUaugaCGUGCucGCGaACACCAUc -3' miRNA: 3'- cgCCCGUa--GUA----GCACG--UGC-UGUGGUA- -5' |
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23359 | 3' | -55.2 | NC_005259.1 | + | 53678 | 0.66 | 0.76003 |
Target: 5'- cGCGGGCAUCG-CGaugGUGCGAC-CgGUc -3' miRNA: 3'- -CGCCCGUAGUaGCa--CGUGCUGuGgUA- -5' |
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23359 | 3' | -55.2 | NC_005259.1 | + | 8213 | 0.66 | 0.769122 |
Target: 5'- uCGGGCAaCAgcgagCGUGCGguggugcCGACACCc- -3' miRNA: 3'- cGCCCGUaGUa----GCACGU-------GCUGUGGua -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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