miRNA: 3'- cgC-CCGU--AGUAgCACGUGCUGUGGUa -5'
miRNA: 3'- -CgCCCGUAGUAGCaCGU-GCUGUGGUa -5'
miRNA: 3'- -CGCCCGuAGUaGC-ACGuGCUGUGGua -5'
miRNA: 3'- -CgCCCGUAGUaGCaCGuGCUGUGGUA- -5'
miRNA: 3'- -CGCCCGUa--GUAGC-ACGuGCU----------GUGGUa -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23359 | 3' | -55.2 | NC_005259.1 | + | 57288 | 0.68 | 0.642923 |
|
Target: 5'- -gGuGGCAccUCGUaGUGCACGACAUCGa -3' miRNA: 3'- cgC-CCGU--AGUAgCACGUGCUGUGGUa -5' |
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| 23359 | 3' | -55.2 | NC_005259.1 | + | 58728 | 0.73 | 0.378818 |
|
Target: 5'- cGgGGGUGUCGUUG-GCAcCGGCACCGg -3' miRNA: 3'- -CgCCCGUAGUAGCaCGU-GCUGUGGUa -5' |
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| 23359 | 3' | -55.2 | NC_005259.1 | + | 58813 | 0.67 | 0.697142 |
|
Target: 5'- gGCGGGC-UCG-CGcUGCuCGGCACCc- -3' miRNA: 3'- -CGCCCGuAGUaGC-ACGuGCUGUGGua -5' |
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| 23359 | 3' | -55.2 | NC_005259.1 | + | 67400 | 0.69 | 0.588449 |
|
Target: 5'- cGgGGGUGUCA-CGgGC-CGACACCAUc -3' miRNA: 3'- -CgCCCGUAGUaGCaCGuGCUGUGGUA- -5' |
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| 23359 | 3' | -55.2 | NC_005259.1 | + | 68704 | 0.67 | 0.675566 |
|
Target: 5'- gGCGGGCAgcgCGUCGgUGCcCGAgaacugggguCGCCGc -3' miRNA: 3'- -CGCCCGUa--GUAGC-ACGuGCU----------GUGGUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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