miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
2336 5' -59.3 NC_001416.1 + 15035 0.66 0.490531
Target:  5'- uGCGCCGUuaaccCGGacGUGCUgacGUCCCGCc -3'
miRNA:   3'- uCGUGGCA-----GCCacCACGG---UAGGGUGc -5'
2336 5' -59.3 NC_001416.1 + 16115 0.68 0.377637
Target:  5'- cAGC-UgGUCuGUGGUGCUGUCCgGCGu -3'
miRNA:   3'- -UCGuGgCAGcCACCACGGUAGGgUGC- -5'
2336 5' -59.3 NC_001416.1 + 32613 0.7 0.276634
Target:  5'- uGGCAUCGUucCGGUGGUGggcguugauggcUgGUCCCGCa -3'
miRNA:   3'- -UCGUGGCA--GCCACCAC------------GgUAGGGUGc -5'
2336 5' -59.3 NC_001416.1 + 5960 1.07 0.000466
Target:  5'- cAGCACCGUCGGUGGUGCCAUCCCACGc -3'
miRNA:   3'- -UCGUGGCAGCCACCACGGUAGGGUGC- -5'
2336 5' -59.3 NC_001416.1 + 9452 0.67 0.386364
Target:  5'- cAGCACC-UCGGUGGUGaaagggCAgagCaCCACGc -3'
miRNA:   3'- -UCGUGGcAGCCACCACg-----GUa--G-GGUGC- -5'
2336 5' -59.3 NC_001416.1 + 12656 0.66 0.460742
Target:  5'- cGGgGCUGUUGGUGGcgGCgCAU-CCGCGu -3'
miRNA:   3'- -UCgUGGCAGCCACCa-CG-GUAgGGUGC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.