Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23361 | 3' | -58.3 | NC_005259.1 | + | 4483 | 0.67 | 0.524333 |
Target: 5'- cGGCgGUggaccauggugaucGCCAcCGCGCcgGGAguggCGCUGCGc -3' miRNA: 3'- -CCGaCA--------------CGGU-GCGCG--UCUa---GCGACGC- -5' |
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23361 | 3' | -58.3 | NC_005259.1 | + | 7429 | 0.66 | 0.621954 |
Target: 5'- cGGCgGUGCC-CGCGCcGcAUgGCaGCGc -3' miRNA: 3'- -CCGaCACGGuGCGCGuC-UAgCGaCGC- -5' |
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23361 | 3' | -58.3 | NC_005259.1 | + | 8132 | 0.68 | 0.496989 |
Target: 5'- cGGCUacggGUGCCcCGCGCcc-UCGUUGCu -3' miRNA: 3'- -CCGA----CACGGuGCGCGucuAGCGACGc -5' |
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23361 | 3' | -58.3 | NC_005259.1 | + | 8655 | 0.67 | 0.568976 |
Target: 5'- aGGCUGUggucgggcuGCCACGCGggcaaCGGGgccgccucgaugUCGCUGuCGa -3' miRNA: 3'- -CCGACA---------CGGUGCGC-----GUCU------------AGCGAC-GC- -5' |
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23361 | 3' | -58.3 | NC_005259.1 | + | 16491 | 0.67 | 0.527408 |
Target: 5'- gGGCgcacCCGCGCGCAuggccgagagcGAggCGCUGCGa -3' miRNA: 3'- -CCGacacGGUGCGCGU-----------CUa-GCGACGC- -5' |
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23361 | 3' | -58.3 | NC_005259.1 | + | 19841 | 0.72 | 0.283299 |
Target: 5'- cGGCUGcUGCCGCGCuCGGuguggCGCUcGCGc -3' miRNA: 3'- -CCGAC-ACGGUGCGcGUCua---GCGA-CGC- -5' |
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23361 | 3' | -58.3 | NC_005259.1 | + | 21625 | 0.76 | 0.16149 |
Target: 5'- aGGCUGUGUCGCGCGgAGuguUCGCcgagGCu -3' miRNA: 3'- -CCGACACGGUGCGCgUCu--AGCGa---CGc -5' |
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23361 | 3' | -58.3 | NC_005259.1 | + | 26416 | 0.67 | 0.558495 |
Target: 5'- cGGCgGUGCCGC-CGUAGAaCGCaccgccgaGCGg -3' miRNA: 3'- -CCGaCACGGUGcGCGUCUaGCGa-------CGC- -5' |
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23361 | 3' | -58.3 | NC_005259.1 | + | 27112 | 0.69 | 0.419257 |
Target: 5'- gGGCUGUGUCAUGCGgAucuccuacgguuuGAgggCGgCUGCGg -3' miRNA: 3'- -CCGACACGGUGCGCgU-------------CUa--GC-GACGC- -5' |
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23361 | 3' | -58.3 | NC_005259.1 | + | 28295 | 0.73 | 0.263152 |
Target: 5'- aGGCcacgGUGCCGCccugcgcaGCGUAGAUCGgUGUGc -3' miRNA: 3'- -CCGa---CACGGUG--------CGCGUCUAGCgACGC- -5' |
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23361 | 3' | -58.3 | NC_005259.1 | + | 36836 | 0.69 | 0.402109 |
Target: 5'- cGGUUGUG-CACGUGCuGAcgccgccCGCUGCGc -3' miRNA: 3'- -CCGACACgGUGCGCGuCUa------GCGACGC- -5' |
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23361 | 3' | -58.3 | NC_005259.1 | + | 45508 | 0.67 | 0.517186 |
Target: 5'- cGCUGUGgCCAcCGCGCcGGuguuggccgccGUgGCUGCGa -3' miRNA: 3'- cCGACAC-GGU-GCGCG-UC-----------UAgCGACGC- -5' |
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23361 | 3' | -58.3 | NC_005259.1 | + | 45931 | 0.69 | 0.448162 |
Target: 5'- aGGCUcUGCC-CGUGCAGAUcccucagcaCGUUGCc -3' miRNA: 3'- -CCGAcACGGuGCGCGUCUA---------GCGACGc -5' |
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23361 | 3' | -58.3 | NC_005259.1 | + | 49350 | 0.66 | 0.601741 |
Target: 5'- aGGCgagagGUGCCGCGCGaugaagaggccggugAGAUCGUUcuugacgccgaGCGa -3' miRNA: 3'- -CCGa----CACGGUGCGCg--------------UCUAGCGA-----------CGC- -5' |
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23361 | 3' | -58.3 | NC_005259.1 | + | 49974 | 0.66 | 0.60068 |
Target: 5'- -aCUGcGCauuGCGCGCacGGAUCGCgUGCGg -3' miRNA: 3'- ccGACaCGg--UGCGCG--UCUAGCG-ACGC- -5' |
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23361 | 3' | -58.3 | NC_005259.1 | + | 50006 | 0.68 | 0.477154 |
Target: 5'- cGGCUGc-CCuCGUGCAcGggCGCUGCGu -3' miRNA: 3'- -CCGACacGGuGCGCGU-CuaGCGACGC- -5' |
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23361 | 3' | -58.3 | NC_005259.1 | + | 60775 | 0.67 | 0.517186 |
Target: 5'- aGGUUGgGCUGCGCGaaCAGAUCaGCcGCGu -3' miRNA: 3'- -CCGACaCGGUGCGC--GUCUAG-CGaCGC- -5' |
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23361 | 3' | -58.3 | NC_005259.1 | + | 66636 | 0.66 | 0.60068 |
Target: 5'- -cCUG-GCCAcCGCuCGGAUUGCUGCc -3' miRNA: 3'- ccGACaCGGU-GCGcGUCUAGCGACGc -5' |
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23361 | 3' | -58.3 | NC_005259.1 | + | 67902 | 0.7 | 0.383683 |
Target: 5'- cGGCUGUggGUCucggcgaggucaaGCGCGUAGGUCGCgaggaugaucuUGCGg -3' miRNA: 3'- -CCGACA--CGG-------------UGCGCGUCUAGCG-----------ACGC- -5' |
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23361 | 3' | -58.3 | NC_005259.1 | + | 67959 | 0.68 | 0.487024 |
Target: 5'- gGGUUGUGCUguagcGCGCGCAGcggCGUgGCc -3' miRNA: 3'- -CCGACACGG-----UGCGCGUCua-GCGaCGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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