miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
23361 5' -57.5 NC_005259.1 + 36544 0.69 0.451339
Target:  5'- -aCGCGGGGUG-AUCUGCGGuGUGGgAu -3'
miRNA:   3'- gaGCGCCCUAUgUAGACGCC-CGUCgU- -5'
23361 5' -57.5 NC_005259.1 + 10170 0.69 0.48073
Target:  5'- aUCuCGGGAaAC-UCUGaGGGCAGCAc -3'
miRNA:   3'- gAGcGCCCUaUGuAGACgCCCGUCGU- -5'
23361 5' -57.5 NC_005259.1 + 20974 0.68 0.497792
Target:  5'- aUCGcCGGGGUgACAaCUGCgugugggccuacucGGGCGGCGa -3'
miRNA:   3'- gAGC-GCCCUA-UGUaGACG--------------CCCGUCGU- -5'
23361 5' -57.5 NC_005259.1 + 41634 0.68 0.511021
Target:  5'- aUgGUGGcGGUGCcgGUCUGCGGGC-GCu -3'
miRNA:   3'- gAgCGCC-CUAUG--UAGACGCCCGuCGu -5'
23361 5' -57.5 NC_005259.1 + 32480 0.67 0.574798
Target:  5'- aCUCGcCGGGcgcgaggugguucuGUAUgaacaccgugagguaGUCgagGCGGGCGGCAg -3'
miRNA:   3'- -GAGC-GCCC--------------UAUG---------------UAGa--CGCCCGUCGU- -5'
23361 5' -57.5 NC_005259.1 + 61411 0.66 0.605801
Target:  5'- cCUCGCGGGcacggACAacacccUUGCGGGCGcGCc -3'
miRNA:   3'- -GAGCGCCCua---UGUa-----GACGCCCGU-CGu -5'
23361 5' -57.5 NC_005259.1 + 25847 0.66 0.61547
Target:  5'- cCUCGCGGauguGGUACucgAUgUGCGGGCgugugccAGCGa -3'
miRNA:   3'- -GAGCGCC----CUAUG---UAgACGCCCG-------UCGU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.