Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23362 | 3' | -57.7 | NC_005259.1 | + | 45780 | 0.66 | 0.653264 |
Target: 5'- -cGGGUGccagcaccgcguUGACggUGUCCUGCUUGGg -3' miRNA: 3'- aaCCCGC------------ACUGgaGCAGGAUGAGCCg -5' |
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23362 | 3' | -57.7 | NC_005259.1 | + | 21961 | 0.66 | 0.653264 |
Target: 5'- --aGGCGUGACCcgcgugggugUCGUUCUccccAC-CGGCa -3' miRNA: 3'- aacCCGCACUGG----------AGCAGGA----UGaGCCG- -5' |
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23362 | 3' | -57.7 | NC_005259.1 | + | 58795 | 0.66 | 0.642574 |
Target: 5'- -cGaGGCgGUGauuGCCUCGgcgggcucgCgCUGCUCGGCa -3' miRNA: 3'- aaC-CCG-CAC---UGGAGCa--------G-GAUGAGCCG- -5' |
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23362 | 3' | -57.7 | NC_005259.1 | + | 31879 | 0.67 | 0.599815 |
Target: 5'- -cGGuCGUGACCUCG-CCggucucaagGCcCGGCg -3' miRNA: 3'- aaCCcGCACUGGAGCaGGa--------UGaGCCG- -5' |
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23362 | 3' | -57.7 | NC_005259.1 | + | 67980 | 0.67 | 0.598749 |
Target: 5'- -aGcGGCGUGGCCaccgccgcgagcgUCGacuugcccUCCUGCggCGGCa -3' miRNA: 3'- aaC-CCGCACUGG-------------AGC--------AGGAUGa-GCCG- -5' |
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23362 | 3' | -57.7 | NC_005259.1 | + | 50287 | 0.67 | 0.589169 |
Target: 5'- -cGGGaacguCGUGcacgcucuGCCacUCGUCgUACUCGGCa -3' miRNA: 3'- aaCCC-----GCAC--------UGG--AGCAGgAUGAGCCG- -5' |
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23362 | 3' | -57.7 | NC_005259.1 | + | 42755 | 0.67 | 0.589169 |
Target: 5'- --aGGCGgu-UCUCGaUCUGCUCGGCg -3' miRNA: 3'- aacCCGCacuGGAGCaGGAUGAGCCG- -5' |
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23362 | 3' | -57.7 | NC_005259.1 | + | 51434 | 0.67 | 0.564825 |
Target: 5'- cUUGGGCGggccacggGGCCUCGcgCCgAuagagcuuguugccCUCGGCg -3' miRNA: 3'- -AACCCGCa-------CUGGAGCa-GGaU--------------GAGCCG- -5' |
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23362 | 3' | -57.7 | NC_005259.1 | + | 59404 | 0.68 | 0.536601 |
Target: 5'- -cGaGCGUGACCUCGcgCaugGCgUCGGCg -3' miRNA: 3'- aaCcCGCACUGGAGCa-Gga-UG-AGCCG- -5' |
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23362 | 3' | -57.7 | NC_005259.1 | + | 61052 | 0.68 | 0.52627 |
Target: 5'- -cGGGCGgcucACCUUGUgCCggGCaUCGGCa -3' miRNA: 3'- aaCCCGCac--UGGAGCA-GGa-UG-AGCCG- -5' |
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23362 | 3' | -57.7 | NC_005259.1 | + | 41213 | 0.68 | 0.516014 |
Target: 5'- --cGGUGUgGAUCgucaCGcCCUGCUCGGCg -3' miRNA: 3'- aacCCGCA-CUGGa---GCaGGAUGAGCCG- -5' |
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23362 | 3' | -57.7 | NC_005259.1 | + | 55713 | 0.68 | 0.516014 |
Target: 5'- aUGGccGCGaGcACCUCGcCCUugUUGGCa -3' miRNA: 3'- aACC--CGCaC-UGGAGCaGGAugAGCCG- -5' |
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23362 | 3' | -57.7 | NC_005259.1 | + | 18963 | 0.68 | 0.495752 |
Target: 5'- aUGGG-GUGACCUCG-CCgcguacaucGCcCGGCa -3' miRNA: 3'- aACCCgCACUGGAGCaGGa--------UGaGCCG- -5' |
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23362 | 3' | -57.7 | NC_005259.1 | + | 49649 | 0.71 | 0.333433 |
Target: 5'- --cGGCGauggcGGCCUCGauacgcgCCUGCUCGGCc -3' miRNA: 3'- aacCCGCa----CUGGAGCa------GGAUGAGCCG- -5' |
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23362 | 3' | -57.7 | NC_005259.1 | + | 58219 | 0.73 | 0.281718 |
Target: 5'- -aGGGUcaGUGGCCUCGagCUcggGCUCGGUg -3' miRNA: 3'- aaCCCG--CACUGGAGCagGA---UGAGCCG- -5' |
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23362 | 3' | -57.7 | NC_005259.1 | + | 60128 | 0.74 | 0.224837 |
Target: 5'- --cGGCG--GCCUCGUCCUGCgCGGCc -3' miRNA: 3'- aacCCGCacUGGAGCAGGAUGaGCCG- -5' |
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23362 | 3' | -57.7 | NC_005259.1 | + | 29456 | 0.74 | 0.242631 |
Target: 5'- ---aGCGUGACCUUG-CCgcccgGCUCGGCa -3' miRNA: 3'- aaccCGCACUGGAGCaGGa----UGAGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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