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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23367 | 3' | -50.8 | NC_005259.1 | + | 10765 | 0.66 | 0.94893 |
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Target: 5'- --aUCAUGUCGGGCggGUCAucgaggUGGGCCg -3' miRNA: 3'- cgaAGUGUAGUUCGggCAGU------ACCUGG- -5' |
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| 23367 | 3' | -50.8 | NC_005259.1 | + | 27444 | 0.68 | 0.872019 |
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Target: 5'- cGCgUCGCAU-GAGCUCGUCGcgcgggaaauugGGGCCg -3' miRNA: 3'- -CGaAGUGUAgUUCGGGCAGUa-----------CCUGG- -5' |
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| 23367 | 3' | -50.8 | NC_005259.1 | + | 41001 | 0.69 | 0.839976 |
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Target: 5'- gGCUUUAUggAUCGGGCCgGUCcacaguGUGGugCc -3' miRNA: 3'- -CGAAGUG--UAGUUCGGgCAG------UACCugG- -5' |
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| 23367 | 3' | -50.8 | NC_005259.1 | + | 56757 | 0.7 | 0.792992 |
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Target: 5'- ---cCACGUguGGCCCaUCggGGACCg -3' miRNA: 3'- cgaaGUGUAguUCGGGcAGuaCCUGG- -5' |
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| 23367 | 3' | -50.8 | NC_005259.1 | + | 15697 | 0.7 | 0.783036 |
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Target: 5'- --cUCAgAUCGAGCCgGUCAcgaUGG-CCa -3' miRNA: 3'- cgaAGUgUAGUUCGGgCAGU---ACCuGG- -5' |
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| 23367 | 3' | -50.8 | NC_005259.1 | + | 2162 | 0.74 | 0.609886 |
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Target: 5'- aGCg--GCGUCGAGCCgCcgguugccgagGUCAUGGGCCg -3' miRNA: 3'- -CGaagUGUAGUUCGG-G-----------CAGUACCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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