Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23368 | 5' | -55.2 | NC_005259.1 | + | 28752 | 0.66 | 0.789968 |
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Target: 5'- -cGCCcgagcGUGAGaUCGUcGCCGGUGcCGa -3' miRNA: 3'- gaCGG-----CACUCaAGCGuCGGCUACaGC- -5' |
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| 23368 | 5' | -55.2 | NC_005259.1 | + | 8701 | 0.66 | 0.770381 |
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Target: 5'- -cGCUGUcGAGgucgUCGCGGUCaagcGUGUCGg -3' miRNA: 3'- gaCGGCA-CUCa---AGCGUCGGc---UACAGC- -5' |
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| 23368 | 5' | -55.2 | NC_005259.1 | + | 58198 | 0.67 | 0.739994 |
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Target: 5'- -cGCCGUGGGcuu---GCCGGUGUCa -3' miRNA: 3'- gaCGGCACUCaagcguCGGCUACAGc -5' |
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| 23368 | 5' | -55.2 | NC_005259.1 | + | 46040 | 0.67 | 0.70866 |
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Target: 5'- uCUG-CGUGAccUCGUAGCCGAUGg-- -3' miRNA: 3'- -GACgGCACUcaAGCGUCGGCUACagc -5' |
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| 23368 | 5' | -55.2 | NC_005259.1 | + | 42944 | 0.68 | 0.68739 |
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Target: 5'- -cGCCGUGGGcgagGCcGCCGuUGUCGa -3' miRNA: 3'- gaCGGCACUCaag-CGuCGGCuACAGC- -5' |
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| 23368 | 5' | -55.2 | NC_005259.1 | + | 32988 | 0.68 | 0.68739 |
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Target: 5'- aUGCCGaGGGUgcCGCcacGcCCGAUGUCGg -3' miRNA: 3'- gACGGCaCUCAa-GCGu--C-GGCUACAGC- -5' |
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| 23368 | 5' | -55.2 | NC_005259.1 | + | 8191 | 0.68 | 0.655126 |
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Target: 5'- cCUGCCGUuua--CGCGcCCGAUGUCGg -3' miRNA: 3'- -GACGGCAcucaaGCGUcGGCUACAGC- -5' |
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| 23368 | 5' | -55.2 | NC_005259.1 | + | 44157 | 0.7 | 0.537112 |
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Target: 5'- gUGCgGUcGAGcagCGC-GCCGGUGUCGg -3' miRNA: 3'- gACGgCA-CUCaa-GCGuCGGCUACAGC- -5' |
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| 23368 | 5' | -55.2 | NC_005259.1 | + | 63128 | 0.7 | 0.52666 |
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Target: 5'- -gGUgGUGAGgUCGUAGCCGcUGUCc -3' miRNA: 3'- gaCGgCACUCaAGCGUCGGCuACAGc -5' |
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| 23368 | 5' | -55.2 | NC_005259.1 | + | 46302 | 0.71 | 0.474676 |
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Target: 5'- -cGCCGUGGcugcguuGUUCGCGGCCccGAggGUCGc -3' miRNA: 3'- gaCGGCACU-------CAAGCGUCGG--CUa-CAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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