Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23369 | 3' | -55.3 | NC_005259.1 | + | 62711 | 0.66 | 0.756949 |
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Target: 5'- cGGcgaucAGGCGGgGgaucUCGCUCGAccgGACCGGc -3' miRNA: 3'- uCC-----UUCGCCgC----AGCGAGUUa--CUGGCU- -5' |
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| 23369 | 3' | -55.3 | NC_005259.1 | + | 47939 | 0.67 | 0.735425 |
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Target: 5'- uGGAAGCcgaugacGGCGgUGC-CGAUGGCCGc -3' miRNA: 3'- uCCUUCG-------CCGCaGCGaGUUACUGGCu -5' |
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| 23369 | 3' | -55.3 | NC_005259.1 | + | 40899 | 0.67 | 0.68365 |
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Target: 5'- cGGGGucGGCaGCGUcccaCGCUUggUGAUCGGa -3' miRNA: 3'- -UCCU--UCGcCGCA----GCGAGuuACUGGCU- -5' |
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| 23369 | 3' | -55.3 | NC_005259.1 | + | 41635 | 0.67 | 0.681504 |
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Target: 5'- uGGuGGCGGUGccggucugcgggCGCUCGGUGAgCGGg -3' miRNA: 3'- uCCuUCGCCGCa-----------GCGAGUUACUgGCU- -5' |
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| 23369 | 3' | -55.3 | NC_005259.1 | + | 61219 | 0.69 | 0.618804 |
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Target: 5'- cAGGAAGCGGCGgugugCGCaccgagaacUCGAUcucggGugCGAg -3' miRNA: 3'- -UCCUUCGCCGCa----GCG---------AGUUA-----CugGCU- -5' |
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| 23369 | 3' | -55.3 | NC_005259.1 | + | 54082 | 0.69 | 0.607981 |
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Target: 5'- gAGGucGGUGGCGUCGCgCAuc-ACCGGg -3' miRNA: 3'- -UCCu-UCGCCGCAGCGaGUuacUGGCU- -5' |
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| 23369 | 3' | -55.3 | NC_005259.1 | + | 16519 | 0.7 | 0.533243 |
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Target: 5'- gAGGcgcuGCGaGCGUCGCUCAAggGugCGc -3' miRNA: 3'- -UCCuu--CGC-CGCAGCGAGUUa-CugGCu -5' |
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| 23369 | 3' | -55.3 | NC_005259.1 | + | 27403 | 0.72 | 0.405194 |
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Target: 5'- cGGcAGCGGCGgcaGCUCGGUGcCCGc -3' miRNA: 3'- uCCuUCGCCGCag-CGAGUUACuGGCu -5' |
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| 23369 | 3' | -55.3 | NC_005259.1 | + | 64026 | 0.73 | 0.369833 |
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Target: 5'- gAGGc-GCGGCGagGC-CGGUGACCGGa -3' miRNA: 3'- -UCCuuCGCCGCagCGaGUUACUGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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