Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio![]() |
P value |
Predicted miRNA align pattern | |||||||
2337 | 5' | -50 | NC_001416.1 | + | 6951 | 1.12 | 0.002491 |
Target: 5'- uCCACGUACUGUCCGGAAUACACGACGa -3' miRNA: 3'- -GGUGCAUGACAGGCCUUAUGUGCUGC- -5' |
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2337 | 5' | -50 | NC_001416.1 | + | 2852 | 0.71 | 0.770795 |
Target: 5'- cCCACccuuCUGgggCCGGAcgGCAUGACa -3' miRNA: 3'- -GGUGcau-GACa--GGCCUuaUGUGCUGc -5' |
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2337 | 5' | -50 | NC_001416.1 | + | 4749 | 0.68 | 0.88865 |
Target: 5'- cCCGCGUccgGCUGaccagcgugCCGGAcaccggcaGCACGGCGa -3' miRNA: 3'- -GGUGCA---UGACa--------GGCCUua------UGUGCUGC- -5' |
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2337 | 5' | -50 | NC_001416.1 | + | 13249 | 0.68 | 0.903091 |
Target: 5'- gCCACgGUACUGgagucguuUCUGGAAgaGCACGGgGg -3' miRNA: 3'- -GGUG-CAUGAC--------AGGCCUUa-UGUGCUgC- -5' |
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2337 | 5' | -50 | NC_001416.1 | + | 39676 | 0.66 | 0.949371 |
Target: 5'- uUCGuCGUACUGuUCCGGcauguuGUugGCGAUc -3' miRNA: 3'- -GGU-GCAUGAC-AGGCCu-----UAugUGCUGc -5' |
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2337 | 5' | -50 | NC_001416.1 | + | 19403 | 0.66 | 0.939535 |
Target: 5'- uCCAuUGUACUG-CCGGAccAC-CGACu -3' miRNA: 3'- -GGU-GCAUGACaGGCCUuaUGuGCUGc -5' |
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2337 | 5' | -50 | NC_001416.1 | + | 27993 | 0.66 | 0.953865 |
Target: 5'- aCCuuGaUACUGUgCCGGAugaaagcgGUuCGCGACGa -3' miRNA: 3'- -GGugC-AUGACA-GGCCU--------UAuGUGCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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