Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23370 | 5' | -57 | NC_005259.1 | + | 57322 | 0.66 | 0.652562 |
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Target: 5'- gCGGUGUCGGUguggucaugUGCGCuGuCCUCUCAa -3' miRNA: 3'- -GCCGCAGUCGa--------GUGUGuC-GGAGAGUa -5' |
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| 23370 | 5' | -57 | NC_005259.1 | + | 12503 | 0.66 | 0.619946 |
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Target: 5'- aGGCG-UAGC-CGCugGGCCgCUCGg -3' miRNA: 3'- gCCGCaGUCGaGUGugUCGGaGAGUa -5' |
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| 23370 | 5' | -57 | NC_005259.1 | + | 66273 | 0.66 | 0.619946 |
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Target: 5'- gGGCGgCGGCUCACGCcGCCa----- -3' miRNA: 3'- gCCGCaGUCGAGUGUGuCGGagagua -5' |
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| 23370 | 5' | -57 | NC_005259.1 | + | 8334 | 0.67 | 0.598239 |
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Target: 5'- aCGGCGaUCAGCcggUCACGCugcuGCCgguaucgCUCGg -3' miRNA: 3'- -GCCGC-AGUCG---AGUGUGu---CGGa------GAGUa -5' |
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| 23370 | 5' | -57 | NC_005259.1 | + | 42552 | 0.68 | 0.534048 |
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Target: 5'- uGGCGggCAGCUCGgcgaGCAGCCggUCGUc -3' miRNA: 3'- gCCGCa-GUCGAGUg---UGUCGGagAGUA- -5' |
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| 23370 | 5' | -57 | NC_005259.1 | + | 41128 | 0.68 | 0.534048 |
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Target: 5'- gCGGUGcCcGCgUCGCGCAGCCUCa--- -3' miRNA: 3'- -GCCGCaGuCG-AGUGUGUCGGAGagua -5' |
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| 23370 | 5' | -57 | NC_005259.1 | + | 54105 | 0.68 | 0.501821 |
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Target: 5'- cCGGgGUCaacgugaGGCUgACAUAGCCgCUCGUg -3' miRNA: 3'- -GCCgCAG-------UCGAgUGUGUCGGaGAGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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