Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23373 | 3' | -54 | NC_005259.1 | + | 34690 | 0.66 | 0.836434 |
Target: 5'- -gGCGCUGCGGAU-GAUgCCGAC-Ca -3' miRNA: 3'- gaCGUGACGUUUAcCUGgGGCUGuGc -5' |
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23373 | 3' | -54 | NC_005259.1 | + | 23733 | 0.66 | 0.827504 |
Target: 5'- gUGCucACgUGgGAcgGcGACCCCGACGCc -3' miRNA: 3'- gACG--UG-ACgUUuaC-CUGGGGCUGUGc -5' |
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23373 | 3' | -54 | NC_005259.1 | + | 52992 | 0.66 | 0.81837 |
Target: 5'- gUGCAUUGCGGcaauuGCCUCGACugGg -3' miRNA: 3'- gACGUGACGUUuacc-UGGGGCUGugC- -5' |
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23373 | 3' | -54 | NC_005259.1 | + | 46274 | 0.66 | 0.8081 |
Target: 5'- uCUGCGCgagguucUGCAAcagcgGGGCCgCCGugGCu -3' miRNA: 3'- -GACGUG-------ACGUUua---CCUGG-GGCugUGc -5' |
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23373 | 3' | -54 | NC_005259.1 | + | 20853 | 0.67 | 0.789846 |
Target: 5'- -cGC-CUGCAA--GGugUUCGACGCGa -3' miRNA: 3'- gaCGuGACGUUuaCCugGGGCUGUGC- -5' |
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23373 | 3' | -54 | NC_005259.1 | + | 50517 | 0.67 | 0.779997 |
Target: 5'- gUGCGCUGC------ACCUCGACGCGc -3' miRNA: 3'- gACGUGACGuuuaccUGGGGCUGUGC- -5' |
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23373 | 3' | -54 | NC_005259.1 | + | 51280 | 0.67 | 0.779997 |
Target: 5'- gUGCGCgGCAAGcGGcaGCgCCCGACAUc -3' miRNA: 3'- gACGUGaCGUUUaCC--UG-GGGCUGUGc -5' |
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23373 | 3' | -54 | NC_005259.1 | + | 17034 | 0.67 | 0.779004 |
Target: 5'- -gGCGCUGgcaucgaCAAccGcGACCCCGAgGCGg -3' miRNA: 3'- gaCGUGAC-------GUUuaC-CUGGGGCUgUGC- -5' |
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23373 | 3' | -54 | NC_005259.1 | + | 32599 | 0.68 | 0.718106 |
Target: 5'- -cGCGCgUGCA---GGACgCCGACACc -3' miRNA: 3'- gaCGUG-ACGUuuaCCUGgGGCUGUGc -5' |
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23373 | 3' | -54 | NC_005259.1 | + | 10036 | 0.68 | 0.70743 |
Target: 5'- --cCACgaccuCGAAUGaGACCCCGACACa -3' miRNA: 3'- gacGUGac---GUUUAC-CUGGGGCUGUGc -5' |
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23373 | 3' | -54 | NC_005259.1 | + | 13206 | 0.69 | 0.642217 |
Target: 5'- -aGCACaGCGgggccagcGGUGGGCCUCGAC-CGg -3' miRNA: 3'- gaCGUGaCGU--------UUACCUGGGGCUGuGC- -5' |
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23373 | 3' | -54 | NC_005259.1 | + | 65782 | 0.7 | 0.631257 |
Target: 5'- -cGCAgUGCGccAUGG-CCUCGACGCGc -3' miRNA: 3'- gaCGUgACGUu-UACCuGGGGCUGUGC- -5' |
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23373 | 3' | -54 | NC_005259.1 | + | 24791 | 0.7 | 0.576649 |
Target: 5'- aUGCGCaGCAGGgccaagaccgaGGuGCCCCGGCACGc -3' miRNA: 3'- gACGUGaCGUUUa----------CC-UGGGGCUGUGC- -5' |
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23373 | 3' | -54 | NC_005259.1 | + | 18202 | 0.71 | 0.533721 |
Target: 5'- -cGCACccaaGCAGGUcGACCCCGAuCACGc -3' miRNA: 3'- gaCGUGa---CGUUUAcCUGGGGCU-GUGC- -5' |
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23373 | 3' | -54 | NC_005259.1 | + | 50024 | 0.71 | 0.523158 |
Target: 5'- -gGCGCUGCGuaauccgcuGUGG-CCUCGACACc -3' miRNA: 3'- gaCGUGACGUu--------UACCuGGGGCUGUGc -5' |
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23373 | 3' | -54 | NC_005259.1 | + | 27357 | 0.73 | 0.413507 |
Target: 5'- -cGUACUGaucgggcuUGGugCCCGGCACGg -3' miRNA: 3'- gaCGUGACguuu----ACCugGGGCUGUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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