Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23373 | 5' | -52.3 | NC_005259.1 | + | 42142 | 0.66 | 0.891252 |
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Target: 5'- -gUACGUCGCGcccGCgUUGGUGCacGCg -3' miRNA: 3'- cuGUGUAGCGCa--CGaAACCACGcaCG- -5' |
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| 23373 | 5' | -52.3 | NC_005259.1 | + | 19092 | 0.67 | 0.842863 |
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Target: 5'- cGACAagcugCGCGaacucaccgGCgcUGGUGUGUGCg -3' miRNA: 3'- -CUGUgua--GCGCa--------CGaaACCACGCACG- -5' |
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| 23373 | 5' | -52.3 | NC_005259.1 | + | 66136 | 0.67 | 0.842863 |
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Target: 5'- aGGC-CAUCagacgGCGUGCgUUGGcauccUGUGUGCg -3' miRNA: 3'- -CUGuGUAG-----CGCACGaAACC-----ACGCACG- -5' |
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| 23373 | 5' | -52.3 | NC_005259.1 | + | 49987 | 0.68 | 0.815511 |
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Target: 5'- cGCACGgaUCGCGUGCggacGGcUGCccucGUGCa -3' miRNA: 3'- cUGUGU--AGCGCACGaaa-CC-ACG----CACG- -5' |
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| 23373 | 5' | -52.3 | NC_005259.1 | + | 60242 | 0.72 | 0.569668 |
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Target: 5'- uGGCACGcUUGCGUGCg--GGUGCaagGCu -3' miRNA: 3'- -CUGUGU-AGCGCACGaaaCCACGca-CG- -5' |
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| 23373 | 5' | -52.3 | NC_005259.1 | + | 66788 | 0.73 | 0.515626 |
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Target: 5'- --aACGUCGCGaGCgcguaaucgaUGGUGCGUGCc -3' miRNA: 3'- cugUGUAGCGCaCGaa--------ACCACGCACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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