miRNA: 3'- -CCGGUGGCUG-GACUa---GAGCGa-CGGGC- -5'
miRNA: 3'- ccGGUGGCUGGACuAGa-GCGACGGgc -5'
miRNA: 3'- -CCGGUGGC--UGGACU----AG---------------AGCGACgGGC- -5'
Results 61 - 63 of 63 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23377 | 5' | -59.5 | NC_005259.1 | + | 22443 | 0.73 | 0.232451 |
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Target: 5'- gGGCCAUCGACuCUGGcuacCUCGUgcGCCCGc -3' miRNA: 3'- -CCGGUGGCUG-GACUa---GAGCGa-CGGGC- -5' |
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| 23377 | 5' | -59.5 | NC_005259.1 | + | 1827 | 0.73 | 0.221123 |
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Target: 5'- -cCCGCCGACCUGcUCgcCGCUGCCg- -3' miRNA: 3'- ccGGUGGCUGGACuAGa-GCGACGGgc -5' |
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| 23377 | 5' | -59.5 | NC_005259.1 | + | 63616 | 0.74 | 0.187038 |
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Target: 5'- cGGCCACCGacGCCUGAuagaUCaucguggcgagcugcUCGCUGgCCGg -3' miRNA: 3'- -CCGGUGGC--UGGACU----AG---------------AGCGACgGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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