Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23378 | 5' | -56.3 | NC_005259.1 | + | 801 | 0.66 | 0.7385 |
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Target: 5'- -gGGUGGcCAAGGgGccggGUGUCGAGAc -3' miRNA: 3'- ugCCGCCaGUUCUgCa---CGCAGCUCUa -5' |
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| 23378 | 5' | -56.3 | NC_005259.1 | + | 56048 | 0.66 | 0.686029 |
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Target: 5'- gGCGGCugcgGGUacuuGAUG-GCGUCGAGAc -3' miRNA: 3'- -UGCCG----CCAguu-CUGCaCGCAGCUCUa -5' |
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| 23378 | 5' | -56.3 | NC_005259.1 | + | 44154 | 0.67 | 0.643094 |
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Target: 5'- aACGuGCGGUCGAGcagcGCGccgGUGUCgGAGAa -3' miRNA: 3'- -UGC-CGCCAGUUC----UGCa--CGCAG-CUCUa -5' |
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| 23378 | 5' | -56.3 | NC_005259.1 | + | 63309 | 0.68 | 0.62153 |
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Target: 5'- aGCGG-GGUCAGGAUGU-CGUUGuAGAUc -3' miRNA: 3'- -UGCCgCCAGUUCUGCAcGCAGC-UCUA- -5' |
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| 23378 | 5' | -56.3 | NC_005259.1 | + | 7833 | 0.68 | 0.578577 |
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Target: 5'- gGCGGCGGUCAccccuuacgGGACGgaccGCGcCGAc-- -3' miRNA: 3'- -UGCCGCCAGU---------UCUGCa---CGCaGCUcua -5' |
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| 23378 | 5' | -56.3 | NC_005259.1 | + | 8445 | 0.68 | 0.567925 |
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Target: 5'- cCGGUGGUCucGGUGUG-GUCGAGGUa -3' miRNA: 3'- uGCCGCCAGuuCUGCACgCAGCUCUA- -5' |
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| 23378 | 5' | -56.3 | NC_005259.1 | + | 14074 | 0.7 | 0.465245 |
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Target: 5'- cACGGCGGUgGccuACGUGCGcagCGAGGUc -3' miRNA: 3'- -UGCCGCCAgUuc-UGCACGCa--GCUCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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