Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23379 | 5' | -61.9 | NC_005259.1 | + | 5431 | 0.69 | 0.280265 |
Target: 5'- uCGaGGUCUG-CGCcCGCUGCCGCCGa -3' miRNA: 3'- -GCcCCAGACaGCGcGUGGCGGUGGUa -5' |
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23379 | 5' | -61.9 | NC_005259.1 | + | 5908 | 0.66 | 0.421685 |
Target: 5'- uCGaGGUCgagGUgcUGCGCACCGUCgACCGUa -3' miRNA: 3'- -GCcCCAGa--CA--GCGCGUGGCGG-UGGUA- -5' |
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23379 | 5' | -61.9 | NC_005259.1 | + | 6211 | 0.72 | 0.188614 |
Target: 5'- gCGGGGUgaGcCGcCGCGCCGaCACCGUg -3' miRNA: 3'- -GCCCCAgaCaGC-GCGUGGCgGUGGUA- -5' |
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23379 | 5' | -61.9 | NC_005259.1 | + | 6250 | 0.71 | 0.203519 |
Target: 5'- aGuGGGUCgauaUCcCGCACCGCCGCCGc -3' miRNA: 3'- gC-CCCAGac--AGcGCGUGGCGGUGGUa -5' |
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23379 | 5' | -61.9 | NC_005259.1 | + | 8550 | 0.66 | 0.430714 |
Target: 5'- uCGGcGGUgUagcccaugcGcCGCaGCACCGCCACCGc -3' miRNA: 3'- -GCC-CCAgA---------CaGCG-CGUGGCGGUGGUa -5' |
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23379 | 5' | -61.9 | NC_005259.1 | + | 12308 | 0.76 | 0.09022 |
Target: 5'- gCGGGcaaUGUCgguGCGCACCGCCGCCAg -3' miRNA: 3'- -GCCCcagACAG---CGCGUGGCGGUGGUa -5' |
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23379 | 5' | -61.9 | NC_005259.1 | + | 18623 | 0.66 | 0.41277 |
Target: 5'- aGcGGUgUGcCGCGCAUCGCC-CCGUu -3' miRNA: 3'- gCcCCAgACaGCGCGUGGCGGuGGUA- -5' |
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23379 | 5' | -61.9 | NC_005259.1 | + | 27479 | 0.72 | 0.17024 |
Target: 5'- cCGGGGUCggUGUgGC-CACCGCC-CCAc -3' miRNA: 3'- -GCCCCAG--ACAgCGcGUGGCGGuGGUa -5' |
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23379 | 5' | -61.9 | NC_005259.1 | + | 28012 | 0.71 | 0.203519 |
Target: 5'- gCGGGGUCgccgggGUUGCGCGCguugGCCAgCCAc -3' miRNA: 3'- -GCCCCAGa-----CAGCGCGUGg---CGGU-GGUa -5' |
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23379 | 5' | -61.9 | NC_005259.1 | + | 33054 | 0.69 | 0.29185 |
Target: 5'- cCGGGGUCgcUCGCGUGCUucugguauccgcugGCCACCu- -3' miRNA: 3'- -GCCCCAGacAGCGCGUGG--------------CGGUGGua -5' |
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23379 | 5' | -61.9 | NC_005259.1 | + | 35501 | 0.66 | 0.403973 |
Target: 5'- -uGGGUUggccccGUUGC-CGCCGCCGCCGg -3' miRNA: 3'- gcCCCAGa-----CAGCGcGUGGCGGUGGUa -5' |
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23379 | 5' | -61.9 | NC_005259.1 | + | 36422 | 0.68 | 0.308114 |
Target: 5'- -aGGGUCaugGUCuGCGCAgCGUCGCCGa -3' miRNA: 3'- gcCCCAGa--CAG-CGCGUgGCGGUGGUa -5' |
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23379 | 5' | -61.9 | NC_005259.1 | + | 37227 | 0.69 | 0.286352 |
Target: 5'- cCGGGGcgCUGgccCGUGCcggauugGCUGCCGCCGUu -3' miRNA: 3'- -GCCCCa-GACa--GCGCG-------UGGCGGUGGUA- -5' |
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23379 | 5' | -61.9 | NC_005259.1 | + | 37627 | 0.66 | 0.439856 |
Target: 5'- uGGGGaugaugugCUGccCGaugacCGCACCGCCGCCGc -3' miRNA: 3'- gCCCCa-------GACa-GC-----GCGUGGCGGUGGUa -5' |
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23379 | 5' | -61.9 | NC_005259.1 | + | 40817 | 0.66 | 0.439856 |
Target: 5'- uCGGGGUCga-CGCGCACgauggGCCGCg-- -3' miRNA: 3'- -GCCCCAGacaGCGCGUGg----CGGUGgua -5' |
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23379 | 5' | -61.9 | NC_005259.1 | + | 43063 | 0.66 | 0.439856 |
Target: 5'- aCGGGGUCgGUgaggaaCGCGaGCCcguuGCCGCCGa -3' miRNA: 3'- -GCCCCAGaCA------GCGCgUGG----CGGUGGUa -5' |
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23379 | 5' | -61.9 | NC_005259.1 | + | 45395 | 0.75 | 0.111841 |
Target: 5'- uCGGGGUCgccgccgagCGCGCugaugGCCGCCGCCGc -3' miRNA: 3'- -GCCCCAGaca------GCGCG-----UGGCGGUGGUa -5' |
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23379 | 5' | -61.9 | NC_005259.1 | + | 46327 | 0.78 | 0.066893 |
Target: 5'- cCGaGGGUCgcGUCGCGCACCGCUugUAg -3' miRNA: 3'- -GC-CCCAGa-CAGCGCGUGGCGGugGUa -5' |
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23379 | 5' | -61.9 | NC_005259.1 | + | 58692 | 0.77 | 0.074613 |
Target: 5'- gCGGGGUCggcucGUCGcCGcCGCCGUCACCAUc -3' miRNA: 3'- -GCCCCAGa----CAGC-GC-GUGGCGGUGGUA- -5' |
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23379 | 5' | -61.9 | NC_005259.1 | + | 58727 | 0.72 | 0.17024 |
Target: 5'- cCGGGGg-UGUCGUugGCACCGgCACCGg -3' miRNA: 3'- -GCCCCagACAGCG--CGUGGCgGUGGUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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