miRNA: 3'- -CCACgACUgACUUaGUaCCACUUGAG- -5'
miRNA: 3'- cCACGAC--UGACUuaguACCACUUGAG- -5'
miRNA: 3'- -CCACGACuGACUUA------GUACCaCUUGAg -5'
miRNA: 3'- ccACGACUGACU--UAGUA--------CCAcUUGAG- -5'
miRNA: 3'- -CCACGACUGACUUAGUACCACUUGAG- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2338 | 5' | -48.4 | NC_001416.1 | + | 19725 | 0.68 | 0.903117 |
|
Target: 5'- cGGUGgUGG-UGAA-CAcGGUGGGCUCa -3' miRNA: 3'- -CCACgACUgACUUaGUaCCACUUGAG- -5' |
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| 2338 | 5' | -48.4 | NC_001416.1 | + | 24162 | 0.68 | 0.916917 |
|
Target: 5'- cGUGCUGcgGCUGGc---UGGUGAACUUc -3' miRNA: 3'- cCACGAC--UGACUuaguACCACUUGAG- -5' |
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| 2338 | 5' | -48.4 | NC_001416.1 | + | 17140 | 0.67 | 0.940766 |
|
Target: 5'- cGGUGUUG-CUGAAUacagcgUAUGG-GAGCUg -3' miRNA: 3'- -CCACGACuGACUUA------GUACCaCUUGAg -5' |
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| 2338 | 5' | -48.4 | NC_001416.1 | + | 37038 | 0.66 | 0.959669 |
|
Target: 5'- -cUGCUGGCUGAuaAUCAUcaccugcaGGUuGGCUCc -3' miRNA: 3'- ccACGACUGACU--UAGUA--------CCAcUUGAG- -5' |
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| 2338 | 5' | -48.4 | NC_001416.1 | + | 7150 | 1.15 | 0.00179 |
|
Target: 5'- cGGUGCUGACUGAAUCAUGGUGAACUCa -3' miRNA: 3'- -CCACGACUGACUUAGUACCACUUGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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