Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 23383 | 3' | -55.4 | NC_005259.1 | + | 57175 | 0.7 | 0.562106 |
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Target: 5'- cGUCCUCGcCGCcguaGUUCGGCCCGcgaACc -3' miRNA: 3'- -UAGGAGCuGCG----CAAGCUGGGCuagUG- -5' |
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| 23383 | 3' | -55.4 | NC_005259.1 | + | 29229 | 0.72 | 0.44996 |
|
Target: 5'- -gCCUCGGCGCGUU---CCUGAUCAUc -3' miRNA: 3'- uaGGAGCUGCGCAAgcuGGGCUAGUG- -5' |
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| 23383 | 3' | -55.4 | NC_005259.1 | + | 26292 | 0.72 | 0.446105 |
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Target: 5'- -cCCUCGACGgGUacggggaUCGACCCauacauauccucggGGUCGCa -3' miRNA: 3'- uaGGAGCUGCgCA-------AGCUGGG--------------CUAGUG- -5' |
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| 23383 | 3' | -55.4 | NC_005259.1 | + | 62983 | 0.79 | 0.164105 |
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Target: 5'- cGUCCUCG-CGCG-UCGACUCGAUgGCa -3' miRNA: 3'- -UAGGAGCuGCGCaAGCUGGGCUAgUG- -5' |
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| 23383 | 3' | -55.4 | NC_005259.1 | + | 29925 | 0.82 | 0.108965 |
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Target: 5'- -aCCaUCGACGCGUggccgccacgCGGCCCGGUCGCg -3' miRNA: 3'- uaGG-AGCUGCGCAa---------GCUGGGCUAGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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