Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23383 | 3' | -55.4 | NC_005259.1 | + | 55837 | 0.67 | 0.691113 |
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Target: 5'- cGUCCUCGACGCGUgugGACUgaaccguggUGAacUCGCc -3' miRNA: 3'- -UAGGAGCUGCGCAag-CUGG---------GCU--AGUG- -5' |
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| 23383 | 3' | -55.4 | NC_005259.1 | + | 57175 | 0.7 | 0.562106 |
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Target: 5'- cGUCCUCGcCGCcguaGUUCGGCCCGcgaACc -3' miRNA: 3'- -UAGGAGCuGCG----CAAGCUGGGCuagUG- -5' |
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| 23383 | 3' | -55.4 | NC_005259.1 | + | 62036 | 0.67 | 0.712308 |
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Target: 5'- -gUgUCGACGUaGUccUCGGCCCGcUCGCg -3' miRNA: 3'- uaGgAGCUGCG-CA--AGCUGGGCuAGUG- -5' |
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| 23383 | 3' | -55.4 | NC_005259.1 | + | 62983 | 0.79 | 0.164105 |
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Target: 5'- cGUCCUCG-CGCG-UCGACUCGAUgGCa -3' miRNA: 3'- -UAGGAGCuGCGCaAGCUGGGCUAgUG- -5' |
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| 23383 | 3' | -55.4 | NC_005259.1 | + | 63994 | 0.66 | 0.7845 |
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Target: 5'- uUCCUCG-CGCGUgacggcauaccaccgCG-CCCGAggCGCg -3' miRNA: 3'- uAGGAGCuGCGCAa--------------GCuGGGCUa-GUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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