Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23383 | 3' | -55.4 | NC_005259.1 | + | 18971 | 0.69 | 0.58345 |
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Target: 5'- -aCCUCGcCGCGUacaUCG-CCCGG-CACg -3' miRNA: 3'- uaGGAGCuGCGCA---AGCuGGGCUaGUG- -5' |
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| 23383 | 3' | -55.4 | NC_005259.1 | + | 18284 | 0.67 | 0.732164 |
|
Target: 5'- -aCCUCGACGCccgacgagccgccGUUCuGCCCGGUgUGCg -3' miRNA: 3'- uaGGAGCUGCG-------------CAAGcUGGGCUA-GUG- -5' |
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| 23383 | 3' | -55.4 | NC_005259.1 | + | 17027 | 0.67 | 0.7332 |
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Target: 5'- cAUCCUCGGCGCuGgcaUCGAcaaccgcgacCCCGAggcggugCGCg -3' miRNA: 3'- -UAGGAGCUGCG-Ca--AGCU----------GGGCUa------GUG- -5' |
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| 23383 | 3' | -55.4 | NC_005259.1 | + | 13130 | 0.67 | 0.691113 |
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Target: 5'- -aCCagCGcACgGUGUUCGGCUCGAUCAUg -3' miRNA: 3'- uaGGa-GC-UG-CGCAAGCUGGGCUAGUG- -5' |
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| 23383 | 3' | -55.4 | NC_005259.1 | + | 9891 | 0.69 | 0.571688 |
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Target: 5'- uUCCUCGcGCGCGacgagcuUUCGGCCC--UCGCg -3' miRNA: 3'- uAGGAGC-UGCGC-------AAGCUGGGcuAGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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