Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23393 | 5' | -58.3 | NC_005259.1 | + | 54136 | 0.66 | 0.615427 |
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Target: 5'- cGUGacCAGAcgguuUCGAggCCCGGCGaUgGGCAUa -3' miRNA: 3'- -CAC--GUCU-----AGCUa-GGGCCGC-AgCCGUA- -5' |
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| 23393 | 5' | -58.3 | NC_005259.1 | + | 57301 | 0.66 | 0.615427 |
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Target: 5'- aGUGCAcGAcaUCGAgcaCCGGCGgugUCGGUGUg -3' miRNA: 3'- -CACGU-CU--AGCUag-GGCCGC---AGCCGUA- -5' |
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| 23393 | 5' | -58.3 | NC_005259.1 | + | 44156 | 0.66 | 0.572779 |
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Target: 5'- cGUGCGG-UCGAgcagcgCgCCGGUGUCGGa-- -3' miRNA: 3'- -CACGUCuAGCUa-----G-GGCCGCAGCCgua -5' |
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| 23393 | 5' | -58.3 | NC_005259.1 | + | 68786 | 0.67 | 0.520551 |
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Target: 5'- -aGCAGGUCGuacgCCggggCGGUGUCGGUg- -3' miRNA: 3'- caCGUCUAGCua--GG----GCCGCAGCCGua -5' |
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| 23393 | 5' | -58.3 | NC_005259.1 | + | 58966 | 0.67 | 0.500171 |
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Target: 5'- -aGCAGGUCGGgcagcUCgUCGGCGaCGGCGa -3' miRNA: 3'- caCGUCUAGCU-----AG-GGCCGCaGCCGUa -5' |
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| 23393 | 5' | -58.3 | NC_005259.1 | + | 53532 | 0.71 | 0.321001 |
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Target: 5'- -gGCAcGGgcUCGGUgCCCGGUGUCGGCu- -3' miRNA: 3'- caCGU-CU--AGCUA-GGGCCGCAGCCGua -5' |
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| 23393 | 5' | -58.3 | NC_005259.1 | + | 33305 | 0.79 | 0.093694 |
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Target: 5'- cUGCGGcUCGAUCCCGcCGUCGGCGg -3' miRNA: 3'- cACGUCuAGCUAGGGCcGCAGCCGUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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