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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23394 | 3' | -54.4 | NC_005259.1 | + | 4287 | 0.66 | 0.769866 |
|
Target: 5'- --uGCCacgaGCAcGGACCGGCGgcccugcgaUGAGCGc -3' miRNA: 3'- cauCGGaa--CGU-CCUGGCCGU---------ACUUGC- -5' |
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| 23394 | 3' | -54.4 | NC_005259.1 | + | 14561 | 0.7 | 0.58657 |
|
Target: 5'- -aGGCCgaGCgGGGACCGGC--GAACa -3' miRNA: 3'- caUCGGaaCG-UCCUGGCCGuaCUUGc -5' |
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| 23394 | 3' | -54.4 | NC_005259.1 | + | 37275 | 0.69 | 0.597517 |
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Target: 5'- -aGGCCgcccgGCAGGACUuGCAUG-GCGg -3' miRNA: 3'- caUCGGaa---CGUCCUGGcCGUACuUGC- -5' |
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| 23394 | 3' | -54.4 | NC_005259.1 | + | 41766 | 0.68 | 0.707012 |
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Target: 5'- -cAGCCUUGCGcGAacaacaCCGGCAUcGGCGa -3' miRNA: 3'- caUCGGAACGUcCU------GGCCGUAcUUGC- -5' |
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| 23394 | 3' | -54.4 | NC_005259.1 | + | 52269 | 0.68 | 0.696213 |
|
Target: 5'- -gGGCCggGCagacAGGACCGGCccGAuGCGc -3' miRNA: 3'- caUCGGaaCG----UCCUGGCCGuaCU-UGC- -5' |
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| 23394 | 3' | -54.4 | NC_005259.1 | + | 52570 | 0.66 | 0.789804 |
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Target: 5'- aUGGUCga-CcGGACCGGCcUGAGCGu -3' miRNA: 3'- cAUCGGaacGuCCUGGCCGuACUUGC- -5' |
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| 23394 | 3' | -54.4 | NC_005259.1 | + | 66921 | 0.68 | 0.663487 |
|
Target: 5'- -cAGCgCgcggUGCucGACCGGgGUGAGCGg -3' miRNA: 3'- caUCG-Ga---ACGucCUGGCCgUACUUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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