Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23395 | 5' | -59.4 | NC_005259.1 | + | 67165 | 0.66 | 0.582304 |
Target: 5'- --aUGGUGG-CgGCCAUgCCGCCGAu- -3' miRNA: 3'- cccGCCACCaGaCGGUA-GGCGGCUug -5' |
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23395 | 5' | -59.4 | NC_005259.1 | + | 30771 | 0.66 | 0.582304 |
Target: 5'- -cGcCGGUGGcaccgCUGCCGUuguucgcguaCCGCCGGAa -3' miRNA: 3'- ccC-GCCACCa----GACGGUA----------GGCGGCUUg -5' |
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23395 | 5' | -59.4 | NC_005259.1 | + | 42508 | 0.66 | 0.571894 |
Target: 5'- cGGCGGUGuGgccagCaGCCGUCCGgccCCGAuggGCa -3' miRNA: 3'- cCCGCCAC-Ca----GaCGGUAGGC---GGCU---UG- -5' |
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23395 | 5' | -59.4 | NC_005259.1 | + | 32899 | 0.66 | 0.571894 |
Target: 5'- gGGGCGaG-GGUgaGCCAcccggcCCGCuCGGGCg -3' miRNA: 3'- -CCCGC-CaCCAgaCGGUa-----GGCG-GCUUG- -5' |
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23395 | 5' | -59.4 | NC_005259.1 | + | 29196 | 0.66 | 0.571894 |
Target: 5'- gGGGCGGaUGGUUcGCCcccgcaugGUUCGCC-AGCc -3' miRNA: 3'- -CCCGCC-ACCAGaCGG--------UAGGCGGcUUG- -5' |
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23395 | 5' | -59.4 | NC_005259.1 | + | 27992 | 0.66 | 0.571894 |
Target: 5'- uGGGUGGUGcGggUGCCGgggcggggUCGCCGGGg -3' miRNA: 3'- -CCCGCCAC-CagACGGUa-------GGCGGCUUg -5' |
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23395 | 5' | -59.4 | NC_005259.1 | + | 41638 | 0.66 | 0.571894 |
Target: 5'- uGGCGGUgccGGUCUGCgGgcgCuCGgUGAGCg -3' miRNA: 3'- cCCGCCA---CCAGACGgUa--G-GCgGCUUG- -5' |
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23395 | 5' | -59.4 | NC_005259.1 | + | 32699 | 0.66 | 0.570855 |
Target: 5'- cGGGCGGUGGUucguacaCUGgCAcUCUcCCGAGu -3' miRNA: 3'- -CCCGCCACCA-------GACgGU-AGGcGGCUUg -5' |
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23395 | 5' | -59.4 | NC_005259.1 | + | 27475 | 0.66 | 0.561529 |
Target: 5'- gGGGcCGG-GGUCggugugGCCA-CCGCCccACg -3' miRNA: 3'- -CCC-GCCaCCAGa-----CGGUaGGCGGcuUG- -5' |
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23395 | 5' | -59.4 | NC_005259.1 | + | 68902 | 0.66 | 0.551215 |
Target: 5'- aGGCGGUGGUUUGUgGUCaUGaCC-AGCa -3' miRNA: 3'- cCCGCCACCAGACGgUAG-GC-GGcUUG- -5' |
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23395 | 5' | -59.4 | NC_005259.1 | + | 31277 | 0.67 | 0.520651 |
Target: 5'- aGGCGGUGGcgagaUCuUGCCcgGUCUGCgcaaCGAGCu -3' miRNA: 3'- cCCGCCACC-----AG-ACGG--UAGGCG----GCUUG- -5' |
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23395 | 5' | -59.4 | NC_005259.1 | + | 32376 | 0.67 | 0.500646 |
Target: 5'- aGGGCGGgca-CUGCCcgCCGUCGcGAUa -3' miRNA: 3'- -CCCGCCaccaGACGGuaGGCGGC-UUG- -5' |
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23395 | 5' | -59.4 | NC_005259.1 | + | 40443 | 0.67 | 0.49471 |
Target: 5'- uGGUGuGUGGcCUGCCGgugugguugacguaCUGCCGGGCc -3' miRNA: 3'- cCCGC-CACCaGACGGUa-------------GGCGGCUUG- -5' |
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23395 | 5' | -59.4 | NC_005259.1 | + | 55344 | 0.67 | 0.480987 |
Target: 5'- -aGCGGUGGcgaUGCCGaaCGCCuGAGCg -3' miRNA: 3'- ccCGCCACCag-ACGGUagGCGG-CUUG- -5' |
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23395 | 5' | -59.4 | NC_005259.1 | + | 43065 | 0.67 | 0.480987 |
Target: 5'- gGGGuCGGUGaGgaacgCgaGCCcguugCCGCCGAACa -3' miRNA: 3'- -CCC-GCCAC-Ca----Ga-CGGua---GGCGGCUUG- -5' |
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23395 | 5' | -59.4 | NC_005259.1 | + | 33361 | 0.7 | 0.36375 |
Target: 5'- cGGGCGa-GGUCaUGaCUcuccuaccgGUCCGCCGAGCg -3' miRNA: 3'- -CCCGCcaCCAG-AC-GG---------UAGGCGGCUUG- -5' |
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23395 | 5' | -59.4 | NC_005259.1 | + | 26054 | 0.7 | 0.36375 |
Target: 5'- cGGGU--UGGcCaUGCCG-CCGCCGAGCa -3' miRNA: 3'- -CCCGccACCaG-ACGGUaGGCGGCUUG- -5' |
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23395 | 5' | -59.4 | NC_005259.1 | + | 36422 | 0.7 | 0.355585 |
Target: 5'- aGGGUcaUGGUCUGCgCAgcgUCGCCGAGg -3' miRNA: 3'- -CCCGccACCAGACG-GUa--GGCGGCUUg -5' |
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23395 | 5' | -59.4 | NC_005259.1 | + | 38742 | 0.7 | 0.34755 |
Target: 5'- cGGCGGUGGcCcGCCGcaggCCGgUGAGCu -3' miRNA: 3'- cCCGCCACCaGaCGGUa---GGCgGCUUG- -5' |
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23395 | 5' | -59.4 | NC_005259.1 | + | 42808 | 0.7 | 0.34755 |
Target: 5'- cGGCGcgugcacuGUGGUauugCUGCCG-CCGCCGAAa -3' miRNA: 3'- cCCGC--------CACCA----GACGGUaGGCGGCUUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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