Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23397 | 5' | -57.3 | NC_005259.1 | + | 66111 | 0.71 | 0.446416 |
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Target: 5'- ---cGCGCGAcuUCuACGCGCGUCGAGc -3' miRNA: 3'- ggcaCGUGCUccAG-UGCGCGCAGCUC- -5' |
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| 23397 | 5' | -57.3 | NC_005259.1 | + | 52009 | 0.72 | 0.390651 |
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Target: 5'- aCCGgaUGCACGAGcGUCaggccgccgcacuGCGCGCugucgccGUCGAGg -3' miRNA: 3'- -GGC--ACGUGCUC-CAG-------------UGCGCG-------CAGCUC- -5' |
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| 23397 | 5' | -57.3 | NC_005259.1 | + | 64269 | 0.72 | 0.383793 |
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Target: 5'- gCGaGCACGAGuUCGCGCGCcaCGAGa -3' miRNA: 3'- gGCaCGUGCUCcAGUGCGCGcaGCUC- -5' |
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| 23397 | 5' | -57.3 | NC_005259.1 | + | 28006 | 0.72 | 0.357984 |
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Target: 5'- gCCGggGCGgggucgcCGGGGUUGCGCGCGUUGGc -3' miRNA: 3'- -GGCa-CGU-------GCUCCAGUGCGCGCAGCUc -5' |
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| 23397 | 5' | -57.3 | NC_005259.1 | + | 62825 | 0.73 | 0.334964 |
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Target: 5'- gCCGcuUGCccGCGAGGUCgaGCGCGCGguugaCGAGc -3' miRNA: 3'- -GGC--ACG--UGCUCCAG--UGCGCGCa----GCUC- -5' |
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| 23397 | 5' | -57.3 | NC_005259.1 | + | 44134 | 0.73 | 0.305018 |
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Target: 5'- gCUGUGCguugaGCGAGGUgaACGUGCgGUCGAGc -3' miRNA: 3'- -GGCACG-----UGCUCCAg-UGCGCG-CAGCUC- -5' |
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| 23397 | 5' | -57.3 | NC_005259.1 | + | 18860 | 0.74 | 0.290833 |
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Target: 5'- gCCGUGCAac-GGUUGCGCGCGUgGGa -3' miRNA: 3'- -GGCACGUgcuCCAGUGCGCGCAgCUc -5' |
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| 23397 | 5' | -57.3 | NC_005259.1 | + | 36440 | 0.75 | 0.257643 |
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Target: 5'- gCGUcGC-CGAGGUCgAC-CGCGUCGAGg -3' miRNA: 3'- gGCA-CGuGCUCCAG-UGcGCGCAGCUC- -5' |
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| 23397 | 5' | -57.3 | NC_005259.1 | + | 4556 | 0.78 | 0.163191 |
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Target: 5'- gCGgGCACGAGGUCACGCcgauCGUCGGc -3' miRNA: 3'- gGCaCGUGCUCCAGUGCGc---GCAGCUc -5' |
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| 23397 | 5' | -57.3 | NC_005259.1 | + | 9507 | 0.8 | 0.115644 |
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Target: 5'- aCGUGCGCuc-GUCGCGCGCGUCGAc -3' miRNA: 3'- gGCACGUGcucCAGUGCGCGCAGCUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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